二氧化钛纳米管对兔胫骨内种植体旋转扭力的影响

Abstract

Abstract: Objective To evaluate the removal torque, osteoblast adhesion and gene expression of different TiO2 nanotubes implant with the rabbit model. Methods A total of 24 custom-made, screw-shaped, commercially pure titanium implants with length of 6 mm and an outer diameter of 2.5 mm were divided into 3 groups. Group A: the control implant is machined. Group B: eight implants anodized with 30 nm nanotubes. Group C: eight implants anodized with 70 nm nanotubes. Four weeks after the surgery, the rabbits were sacrificed. Subsequently, the leg was stabilized and the implant was removed under reverse torque rotation with a digital torque gauge. Osteoblast adhesion was analyzed with scanning electron microscope. The gene expression of Runt-related transcription factor2 (Runx2)、Insulinlike growth factors (IGF)、collagen-I (col-1) and osteocalcin (OCN) were examined by using real-time PCR. Results The results showed that implants anodized with 70 nm nanotubes could enhance removal torque in rabbit tibia and significantly improve the Runx2、IGF、col-1 and OCN gene expression level(P<0.05). Conclusions The implants with large diameter nantubes achieved favorable osteoblast response and a higher interfacial strength at an early implantation period compared with the machined titanium implants and small diameter nantube implants.

Key words: nanotubes, removal torque, osteoblast, bone gene, rabbit

CLC Number:

R783.1

References

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The removal torque of titania nanotubes in rabbit tibia

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