去甲肾上腺素对FaDu鳞癌细胞系生物学行为的影响

Abstract

Abstract: Objective To investigate the effects of norepinephrine(NE) on thebiological behavior of human pharyngeal squamous cell carcinoma cell line FaDu.MethodsMTT assay was used to evaluate the influence of NEontheproliferation of tumor cells. Transwellinvasion assay was used to evaluate theinfluence of NE on theinvasion of tumor cells.The effects of NE on themigrationof tumor cells wasmeasuredby Transwellmigration assayand wound-healing assay.Additionally, theeffects of the β-adrenergic antagonist propranolol on the NE-induced proliferation, invasion and migrationof tumor cellswere measured.Results10μmol/LNE promotedtheproliferation, invasion and migration of tumor cells significantly(P<0.001). However,pretreatment with 1μmol/L propranolol effectivelyinhibitedthe NE-induced proliferation, invasion and migration(P<0.001). ConclusionNEpromotestheproliferation, invasion and migration of human pharyngeal squamous cell carcinoma cells.These effectscan be suppressed by propranolol.

Key words: human pharyngeal squamous cell carcinoma cell line FaDu, norepinephrine, propranolol, proliferation, invasion, migration

CLC Number:

R739.8

References

[1]Powe DG,Voss MJ,Habashy HO,et al.Alpha- and beta-adrenergic receptor (AR) protein expression is associated with poor clinical outcome in breast cancer: an immunohistochemical study[J].Breast Cancer Res Treat,2011,130(2):457-463
[2]Yamazaki S,Miyoshi N,Kawabata K,et al.Quercetin-3-O-glucuronide inhibits noradrenaline-promoted invasion of MDA-MB-231 human breast cancer cells by blocking beta(2)-adrenergic signaling[J].Arch BiochemBiophys,2014,557:18-27
[3]Kim-Fuchs C,Le CP,Pimentel MA,et al.Chronic stress accelerates pancreatic cancer growth and invasion: a critical role for beta-adrenergic signaling in the pancreatic microenvironment[J].Brain Behav Immun,2014,40:40-47
[4]Shang ZJ,Liu K,de F L.Expression of beta2-adrenergic receptor in oral squamous cell carcinoma[J].J Oral Pathol Med,2009,38(4):371-376
[5]Perez-Sayans M,Somoza-Martin JM,Barros-Angueira F,et al.Activity of beta2-adrenergic receptor in oral squamous cell carcinoma is mediated by overexpression of the ADRBK2 gene: a pilot study[J].Biotech Histochem,2012,87(3):179-186
[6]Bernabe DG,Tamae AC,BiasoliER,et al.Stress hormones increase cell proliferation and regulates interleukin-6 secretion in human oral squamous cell carcinoma cells[J].Brain Behav Immun,2011,25(3):574-583
[7]Lutgendorf SK,Sood AK,Antoni MH.Host factors and cancer progression: biobehavioral signaling pathways and interventions[J].J ClinOncol,2010,28(26):4094-9
[8]Sood AK,Bhatty R,Kamat AA,et al.Stress hormone-mediated invasion of ovarian cancer cells[J].Clin Cancer Res,2006,12(2):369-375
[9]李博，李黎，段泽西，等．儿茶酚胺水平与口腔鳞癌患者精神心理因素变化的关系[J]．上海口腔医学．2013．22(6)：671-675
[10]Tang J,Li Z,Lu L,et al.beta-Adrenergic system,a backstage manipulator regulating tumour progression and drug target in cancer therapy[J].Semin Cancer Biol,2013,23(6 Pt B):533-542
[11]Cole SW,Sood AK.Molecular pathways: beta-adrenergic signaling in cancer[J].Clin Cancer Res,2012,18(5):1201-1206
[12]Bravo-Calderon DM,Oliveira DT,Marana AN,et al.Prognostic significance of beta-2 adrenergic receptor in oral squamous cell carcinoma[J].Cancer Biomark,2011,10(1):51-59
[13]Yang EV,Sood AK,Chen M,et al.Norepinephrine up-regulates the expression of vascular endothelial growth factor,matrix metalloproteinase (MMP)-2,and MMP-9 in nasopharyngeal carcinoma tumor cells[J].Cancer Res,2006,66(21):10357-10364
[14]Wolter NE,Wolter JK,Enepekides DJ,et al.Propranolol as a novel adjunctive treatment for head and neck squamous cell carcinoma[J].J Otolaryngol Head Neck Surg,2012,41(5):334-344
[15]Baker JG,Hill SJ,Summers RJ.Evolution of beta-blockers: from anti-anginal drugs to ligand-directed signalling[J].Trends Pharmacol Sci,2011,32(4):227-234
[16]Barron TI,Connolly RM,Sharp L,et al.Beta blockers and breast cancer mortality: a population- based study[J].J ClinOncol,2011,29(19):2635-2644

Effects of norepinephrine on the biological behavior of human pharyngeal squamous cell carcinoma cell line FaDu

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	. Influences of long non-coding RNA MEG3 on the proliferation and invasion of HNSCC [J]. , 2019, 39(5): 400-403.
[2]	. Transmigration or driftodontics: Analysis of 5 cases of mandibular canines [J]. , 2019, 39(3): 250-253.
[3]	. Inhibitory effect of danshensu on oral squamous cell carcinoma CAL-27 cells in vitro [J]. , 2019, 39(2): 113-116.
[4]	. Influence of nobelitin on the proliferation，migration and invasion of oral squamous cell carcinoma Cal-27 cells [J]. , 2018, 38(6): 481-484.
[5]	. Analysis of GNAS mutations and biological characteristics of bone marrow stromal cells in fibrous dysplasia of the jaws [J]. , 2018, 38(4): 289-294.
[6]	. The protective effects of hyaluronic acid against nicotine in human periodontal ligament fibroblasts [J]. , 2018, 38(4): 314-319.
[7]	. Effect of autophagy on proliferation of osteoblasts in hypoxia [J]. , 2018, 38(10): 876-880.
[8]	. Influence of IL-17 on RAW264.7 cells proliferation and differentiation [J]. , 2018, 38(10): 872-875.
[9]	. Effect of the growth differentiation factor 15 on rat bone marrowderived mesenchymal stem cell proliferation and gene expression [J]. , 2017, 37(9): 778-784.
[10]	. Research progress on the regulatory effect of bFGF in dental pulp stem cell proliferation,migration and differentiation [J]. , 2017, 37(8): 746-750.
[11]	. Effect of delta-like 1 homologue on the proliferation and dontoblastic differentiation in human dental pulp stem cells [J]. , 2017, 37(8): 678-685.
[12]	. Expression and significance of midkine in salivary adenoid cystic carcinoma [J]. , 2017, 37(4): 298-301.
[13]	ZHAO Zhenyan SUN Moyi LI Yun MA Chao JU Jun NI Qianwei GAO Tao REN Yixiong. Effect of Hydroxycamptothecin on proliferation，migration，cellcycle distribution and apoptosis in human pharyngeal squamous carcinoma cell line FaDu [J]. , 2017, 37(2): 102-105.
[14]	Xu yunlong. Progress in clinical application of resin penetration [J]. , 2016, 36(9): 849-851.
[15]	. MicroRNA27b inhibits cell proliferation of oral squamous cell carcinoma through targeting FZD7 [J]. , 2016, 36(3): 200-205.