›› 2016, Vol. 36 ›› Issue (11): 968-971.

• Basic Research • Previous Articles     Next Articles

A study of the production of interleukin-34 in condylar cartilage cells stimulated by TNF-α

  

  • Received:2016-06-03 Revised:2016-07-16 Online:2016-11-28 Published:2016-11-29

Abstract: Objective To address the expression of IL-34 regulated by TNF-α in SD rats mandibular condylar cartilage (MCC) cells and to investigate its regulation and pathogenic role in temporomandibular disorders. Methods The third generation of condylar cartilage cells were taken. A variety of concentrations (0, 1, 10, 20, 50 ng/mL) of TNFα were added to the culture medium for 24h and cells were cultured at increasing timepoints (1, 3, 6, 10, 24 h) in the presence of TNF-α (10 ng/mL). The production of IL34 was measured using enzymelinked immunosorbent assay (ELISA)and IL-34 mRNA levels were determined by realtime reverse transcriptionpolymerase chain reaction (RTPCR). Results ①IL-34 was detected in MCC cells; ②The production of IL-34mRNA was enhanced by TNF-α in a timedependent and dosedependent manner. Conclusion This data provides novel information about the production of IL-34 stimulated by TNF-α in MCC cells.

Key words: interleukin-34, condylar cartilage cells, tumor necrosis factor-α, rat

CLC Number: