›› 2016, Vol. 36 ›› Issue (2): 109-111.

• Basic Research • Previous Articles     Next Articles

The effect of 1064nm Nd:YAG laser irradiation on the migration of oral squamous cell carcinoma CAL-27 cells

  

  • Received:2015-10-21 Revised:2015-11-26 Online:2016-02-28 Published:2016-02-29
  • Contact: wu guoYING E-mail:wuguoying888@163.com

Abstract: Abstract: Objective To investigate the effect of 1064nm Nd:YAG laser irradiation on migration of oral squamous cell carcinoma CAL-27 cells. Methods Squamous carcinoma CAL-27 cells were cultured in vitro, and cells in logarithmic growth phase were inoculated in 24-well plates and irradiated with 1064nm Nd:YAG continuous wave laser for different time intervals (0, 120, 240, 360, 480 s) with different corresponding doses (0, 44.4, 88.8, 133.2, 177.6 J/cm2). After the specimens were scratched, the healing change of those scratches were observed under inverted microscope at 0, 12, 24, 48 h. Results After a low dose of laser irradiation, the wound healing degree were improved, while after a high dose of laser irradiation, the degree of wound healing were reduced. Conclusions The squamous carcinoma CAL-27 cell migration can be stimulated by 1064nm Nd:YAG laser at a low intensity, while it can be inhibited by high intensity laser.

Key words: Nd:YAG laser, squamous carcinoma, cell migration

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