A20对IL-17介导人牙周膜细胞RANKL表达影响

Abstract

Abstract: Abstract: Objective To investigate the potential function of A20 on modulating RANKL expression of the human periodontal ligament cells (hPDLCs) regulated by IL-17. Methods HPDLCs were transfected of A20siRNA and slow virus, and then hPDLCs by suppression/over-expression of A20 were obtained, which were divided into suppression group, control group and over-expression group. Treated with IL-17 (50 ng/ml) for certain time, protein and mRNA levels of RANKL were determined by enzyme-linked immunosorbent assays (ELISA), real-time PCR (RT-PCR) and western blotting. Results Suppression/over-expression of A20 in hPDLCs increased/decreased the expression of RANKL protein compared to the control group. Upon the stimulation of IL-17 (50ng/ml), similar results were observed in the three groups of hPDLCs. Conclusion Our study revealed for the first time that IL-17 induced RANKL expression of hPDLCs may be interfered by A20.

Key words: periodontal ligament cells, IL-17, tumor necrosis factor alpha-induced protein3, RANKL

CLC Number:

R780.2

References

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Effect of A20 on modulating RANKL expression of human periodontal ligament cells (hPDLCs) through IL-17

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