›› 2016, Vol. 36 ›› Issue (9): 774-777.

• Basic Research • Previous Articles     Next Articles

Preparation of gold nanoparticles and research of its biocompatibility in vitro and in vivo

  

  • Received:2016-04-13 Revised:2016-05-27 Online:2016-09-28 Published:2016-10-06

Abstract: Objective To prepare gold nanoparticles(GNPs)and to discuss their biocompatibility preliminarily. Methods GNPs were synthesized by reduction method, the shape and optical properties of which were characterized by transmission electron microscopy (TEM) and UV-Vis. Then, the cell toxicity of GNPs was measured by MTT assay in vitro and H & E stain was used to observe the changes in heart, liver, spleen and kidney tissues between BABL/c mouse which were injected with GNPs and control in vivo. Results The TEM and UV-Vis results showed narrowed distribution of the diameters of spherical gold nanoparticles. In vitro MTT assay and in vivo H & E stain both showed that GNPs were safe and biocompatible with no significant toxicity. Conclusions Gold nanoparticles were successfully produced with good biocompatibility, revealing potential prospects of clinical applications.

Key words: human pharyngeal squamous cell carcinoma(FaDu), gold nanoparticles, cell toxicity, biocompatibility

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