Abstract: Objective To investigate the effect of Ginsenoside Rg3 on proliferation and apoptosis in human salivary adenoid cystic carcinoma cells. Methods SACC-M cells were treated with Ginsenoside Rg3 (10 μg/mL, 50 μg/mL, 100 μg/mL, 150 μg/mL, 200 μg/mL) for 24, 48, 72 h respectively. The inhibition of cell proliferation was detected by MTT assay, and the rate of apoptosis was measured by flow cytometer. The activity of NF-κB was measured by EMSA. Results 10-200 μg/mL Ginsenoside Rg3 could significantly inhibit the proliferation and induced the apoptosis of SACC-M cells. 200 μg/mL Ginsenoside Rg3 could induce the highest rate of apoptosis(88.69%). The results of Real-time PCR proved that Ginsenoside Rg3 could induce the expression level of bax and caspae-3. EMSA assay indicated that Ginsenoside Rg3 could suppress the activity of NF-κB. Conclusion Ginsenoside Rg3 could increase the expression level of bax and caspase-3 through inhabiting theactivity of NF-κB, thus finally suppressing the proliferation and then inducing apoptosis in SACC-M cells.