乏氧对IL-17调控牙周膜细胞RANKL及OPG表达的影响

Abstract

Abstract: Objective To investigate the effect of IL-17 on modulating the expression of RANKL and OPG in human periodontal ligament cells（hPDLCs）under hypoxia. Methods Human PDLCs were incubated in the normoxia (normal oxygen tension) and different hypoxic atmospheres (oxygen volume fraction 10%, 5%, 2%) for 24 hours. After that, cell proliferation assay was determined using CCK-8 technique. The expression levels of RANKL and OPG were determined by western blotting and real-time PCR. Results Human PDLCs proliferation increased in the normoxia and the hypoxic atmosphere of 10% O and 5% O and had a positive correlation with time in a time-dependent manner while the hPDLCs proliferation was inhibited in the hypoxic atmosphere of 2% O. With the decrease of oxygen concentration under hypoxia, OPG mRNA and protein levels were up-regulated at first and then down-regulated meanwhile RANKL mRNA and protein levels were up-regulated after stimulated by hypoxia. Conclusions The relative RANKL/OPG expression ratios increase mostly in a hypoxic atmosphere of 5% O, suggesting that moderate hypoxia can modulate the alveolar remodeling via regulating the expressions of pro-osteoplastic molecules of hPDLCs through IL-17.

Key words: Hypoxia, periodontal ligament cells, IL-17, HIF-1α, RANKL, OPG

CLC Number:

R329.28

References

[1]Majmundar AJ,Wong WJ,Simon MC.Hypoxia-inducible factors and the response to hypoxic stress[J].Mol Cell,2010,40(2):294-309
[2]Belibasakis GN,Bostanci N.The RANKL-OPG system in clinical periodontology[J].J Clin Periodontol,2012,39(3):239-248
[3]Zhao L,Zhou Y,Xu Y,et al.Effect of non-surgical periodontal therapy on the levels of Th17/Th1/Th2 cytokines and their transcription factors in Chinese chronic periodontitis patients[J].J Clin Periodontol,2011,38(6):509-516
[4]Adibrad M,Deyhimi P,Ganjalikhani Hakemi M,et al.Signs of the presence of Th17 cells in chronic periodontal disease[J].Journal of Periodontal Research,2012,47(4):525-531
[5]Eskan MA,Jotwani R,Abe T,et al.The leukocyte integrin antagonist Del-1 inhibits IL-17-mediated inflammatory bone loss[J].Nat Immunol,2012,13(5):465-473
[6]林丹萍，王威栋，李璐，等.IL-17对人牙周膜成纤维细胞RANKL表达的影响[J].口腔医学,2014,34(6):405-408
[7]Moon YM,Yoon BY,Her YM.,et alIL-32 and IL-17 interact and have the potential to aggravate osteoclastogenesis in rheumatoid arthritis[J].. Arthritis Res Ther,2012,14:246-
[8]Patel DD,Lee DM,Kolbinger F,et al.Effect of IL-17A blockade with secukinumab in autoimmune diseases[J].Ann Rheum Dis,2013,72(Suppl 2):i116-i123
[9]Duarte PM,Miranda TS,Lima JA,et al.Expression of Immune-Inflammatory Markers in Sites of Chronic Periodontitis in Patients With Type 2 Diabetes[J].J Periodontol,2012,83(4):426-434
[10]Dutzan N,Gamonal J,Silva A,et al.Over-expression of forkhead box P3 and its association with receptor activator of nuclear factor-κ B ligand,interleukin (IL) -17,IL-10 and transforming growth factor-β during the progression of chronic periodontitis[J].J Clin Periodontol,2009,36(5):396-403
[11]Danping L,Li L,Sun Y,et al.Interleukin-17 regulates the expressions of RANKL and OPG in human periodontal ligament cells via TRAF6/TBK1-JNK/NF-κB pathways[J].Immunology,2014,144(3):472-485
[12]叶宇,俞文伟,陈旭,等.A20对IL-17介导人牙周膜细胞RANKL表达影响[J].口腔医学,2016,36(6):489-493
[13]Buduneli N,Kinane DF.Host-derived diagnostic markers related to soft t destruction and bone degradation in periodontitis[J].J Clin Periodontol,2011,38:85-105
[14]Shi LZ,Wang R,Huang G,et al.HIF1 alpha-dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cells[J].J Exp Med,2011,208(7):1367-1376
[15]Wang H,Flach H,Onizawa M,et al.Negative regulation of Hif1a expression and TH17 differentiation by the hypoxia-regulated microRNA miR-210[J].Nat Immunol,2014,15(4):393-401
[16]Bosco MC,Puppo M,Blengio F,et al.Monocytes and dendritic cells in a hypoxic environment: Spotlights on chemotaxis and migration[J].Immunobiology,2008,213(9/10):733-749
[17]Safronova O,Pluemsampant S,Nakahama K,et al.Regulation of chemokine gene expression by hypoxia via cooperative activation of NF-kappaB and histone deacetylase[J].Int J Biochem Cell Biol,2009,41(11):2270-2280
[18]Dandajena TC,Ihnat MA,Disch B,et al.Hypoxia triggers a HIF-mediated differentiation of peripheral blood mononuclear cells into osteoclasts[J].Orthod Craniofac Res,2012,15(1):1-9
[19]Xu N,Liu H,Qu F,et al.Hypoxia inhibits the differentiation of mesenchymal stem cells into osteoblasts by activation of Notch signaling[J].Exp Mol Pathol,2013,94(1):33-39
[20]Song ZC,Zhou W,Shu R,et al.Hypoxia induces apoptosis and autophagic cell death in human periodontal ligament cells through HIF-1α pathway[J].Cell Prolif,2012,45(3):239-248

Effect of IL-17 on modulating the expression of RANKL and OPG in periodontal ligament cells under hypoxia

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