十字孢碱对口腔鳞状细胞癌CAL27细胞增殖和凋亡的影响

Abstract

Abstract: Abstract: Objective To study the effect of staurosporine (ST) on cell proliferation and apoptosis in oral squamous cell carcinoma cell line CAL27 and to explore the possible mechanisms. Methods After CAL27 cells were treated with different concentrations of ST for different durations, cell proliferation was assessed by CCK-8 method, cell apoptosis was detected by AnnexinV-FITC Apoptosis Detection Kit and flow cytometry, cell cycle distribution was investigated by flow cytometry, and expression of cyclin B1 and Cdk1 proteins was detected by Western blot. Results ST significantly inhibited the proliferation of CAL27 cells (P < 0.05) in a dose- dependent manner and the apoptosis rate of CAL27 cells treated with ST increased significantly compared with the controlled cells (P < 0.05). Treatment with ST induced cell cycle arrest in G2/M phase (P < 0.05). Moreover, ST treatment significantly decreased the expression of cyclin D1 、Cdk4 and Cdk6 proteins and increased the expression of P21 in CAL27 cells (P < 0.05).Conclusion ST significantly inhibits proliferation and induces apoptosis of CAL27 cells possibly by inducing cell cycle arrest in G2/M phase.

Key words: oral squamous cell carcinoma, Staurosporine, cell cycle, apoptosis, CAL27 cells

CLC Number:

R739.85

References

[1]Praefcke GJ,Hofmann K,Dohmen RJ.SUMO playing tag with ubiquitin[J].Trends Biochem Sci,2012,37(1):23-31
[2]Jerjes W,Upile T,Petrie A,et al.Clinicopathological parameters,recurrence,locoregional and distant metastasis in 115 T1-T2 oral squamous cell carcinoma patients[J].Head Neck Oncol,2010,10(2):9-
[3]Friemel J,Foraita R,Gunther K,et al.Pretreatment oral hygiene habits and survival of head and neck squamous cell carcinoma (HNSCC) patients[J].BMC Oral Health,2016,16(1):33-
[4]Griner EM,Kazanietz MG.Protein kinase C and other diacylglycerol effectors in cancer[J].Nat Rev Cancer,2007,7(4):281-294
[5]Francis H,Onori P,Gaudio E,et al.H3 histamine receptor-mediated activation of protein kinase Calpha inhibits the growth of cholangiocarcinoma in vitro and in vivo[J].Mol Cancer Res,2009,7(10):1704-1713
[6]Techasen A,Loilome W,Namwat N,et al.Myristoylated alanine-rich C kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase C-dependent pathway[J].Cancer Sci,2010,101(3):658-665
[7]Bernard B,Fest T,Pretet JL,et al.Staurosporine-induced apoptosis of HPV positive and negative human cervical cancer cells from different points in the cell cycle[J].Cell Death Differ,2001,8(3):234-244
[8]Yadav SS,Prasad C,Prasad SB,et al.Anti-tumor activity of staurosporine in the tumor microenvironment of cervical cancer: An in vitro study[J].Life Sci,2015,133(7):21-28
[9]Fukami K,Inanobe S,Kanemaru K,et al.Phospholipase C is a key enzyme regulating intracellular calcium and modulating the phosphoinositide balance[J].Prog Lipid Res,2010,49(4):429-437
[10]Li X,Marani M,Mannucci R,et al.Overexpression of BCL-X(L) underlies the molecular basis for resistance to staurosporine-induced apoptosis in PC-3 cells[J].Cancer Res,2001,61(4):1699-1706
[11]Khanal G,Hiemstra S,Pappas D.Probing hypoxia-induced staurosporine resistance in prostate cancer cells with a microfluidic culture system[J].Analyst,2014,139(13):3274-3280
[12]Mackay HJ,Twelves CJ.Protein kinase C: a target for anticancer drugs?[J].Endocr Relat Cancer,2003,10(3):389-396
[13]Luna-Ulloa LB,Hernandez-Maqueda JG,Castaneda-Patlan MC,et al.Protein kinase C in Wnt signaling: implications in cancer initiation and progression[J].IUBMB Life,2011,63(10):915-921
[14]Mukherjee A,Roy S,Saha B,et al.Spatio-Temporal Regulation of PKC Isoforms Imparts Signaling Specificity[J].Front Immunol,2016,7:45-
[15]Kim S,Chun S Y,Kwon YS,et al.Crosstalk between Wnt signaling and Phorbol ester-mediated PKC signaling in MCF-7 human breast cancer cells[J].Biomed Pharmacother,2016,77:114-119
[16]Pietenpol JA,Stewart ZA.Cell cycle checkpoint signaling: cell cycle arrest versus apoptosis[J].Toxicology,2002,181-182:475-481
[17]Motokura T,Arnold A.Cyclin D and oncogenesis[J].Curr Opin Genet Dev,1993,3(1):5-10
[18]Pietenpol JA,Stewart ZA.Cell cycle checkpoint signaling: cell cycle arrest versus apoptosis[J].Toxicology,2002,181(12):475-481
[19]Schwartz G K,Shah MA.Targeting the cell cycle: a new approach to cancer therapy[J].J Clin Oncol,2005,23(36):9408-9421

Effect of staurosporine on cell proliferation and apoptosis in oral squamous cell carcinoma cell line CAL27

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	. Advances in the study of factors related to the occurrence and development of oral squamous cell carcinoma mediated by PI3K/AKT pathway [J]. , 2019, 39(6): 552-556.
[2]	. Inhibitory effect of danshensu on oral squamous cell carcinoma CAL-27 cells in vitro [J]. , 2019, 39(2): 113-116.
[3]	. Effect of IL-17 combined with IFN-gamma on the proliferation and apoptosis of RAW264.7 in vitro [J]. , 2019, 39(2): 108-112.
[4]	. Recombinant human lactoferrin inhibits proliferation and induces apoptosis in oral cancer KB cells [J]. , 2018, 35(9): 785-790.
[5]	. A clinicopathological study of the prognosis and metastasis of MMP-14 in oral cancer patients [J]. , 2018, 38(8): 685-689.
[6]	. DNA quantitative analysis of oral squamous cell carcinoma and its clinical significance [J]. , 2018, 38(7): 616-619.
[7]	. Influence of nobelitin on the proliferation，migration and invasion of oral squamous cell carcinoma Cal-27 cells [J]. , 2018, 38(6): 481-484.
[8]	. Non-invasive Human Papilloma Virus Detection of Oral leukoplakia and Squamous Cell Carcinomas [J]. , 2018, 38(6): 528-532.
[9]	. Expression regulation of microRNA and oral squamous cell carcinoma [J]. , 2018, 38(5): 476-480.
[10]	. Effect of Fusobacterium nucleatum on cell proliferation and production of inflammatory factors in KB cells [J]. , 2018, 38(2): 109-113.
[11]	. Research progress of the role of IL-17 in osteoclasts apoptosis [J]. , 2018, 38(11): 1031-1034.
[12]	. Relationship between E-cadherin and MMP-1 expressions and prognosis in oral squamous cell carcinoma [J]. , 2018, 38(1): 39-43.
[13]	chen xin. Current situation of oral squamous cell carcinoma [J]. , 2017, 37(5): 462-465.
[14]	. The expressions of Slug and E-cadherin in oral squamous cell carcinoma [J]. , 2017, 37(3): 214-218.
[15]	ZHAO Zhenyan SUN Moyi LI Yun MA Chao JU Jun NI Qianwei GAO Tao REN Yixiong. Effect of Hydroxycamptothecin on proliferation，migration，cellcycle distribution and apoptosis in human pharyngeal squamous carcinoma cell line FaDu [J]. , 2017, 37(2): 102-105.