IL-17联合IFN-γ体外诱导RAW264.7细胞增殖和凋亡的影响

Abstract

Abstract: Abstract: Objective To investigate the effects of IL-17 combined with IFN-γduring the process of inducing RAW264.7 of mouse osteoclast precursor cell line into mature osteoclasts on the proliferation and apoptosis. Methods The differentiation study model of osteoclastin in vitro was established with 50 ng/mL RANKL and 30 ng/mL M-CSF inducing RAW264.7 of mouse osteoclast precursor cell line. After Raw264.7 cells were cultured for 24 hours, they were divided into three groups: the first group of IL-17, the second group of IFN-γ, and the third group of IL-17 combined with IFN-γ.The cultured osteoclasts were identified by the staining of tartrate-resistance acid phosphatase (TRAP). Viability was determined using cell counting kit-8. Apoptosis was measured with AnnexinV cell apoptosis test. Furthermore, RT-PCR was used to examine the expression difference of mRNA. Results With 50 ng/mL IL-17 combined with different concentrations of IFN-γ, the growth of RAW264.7 was inhibited by the increase of IFN-γin a concentration dependent manner. IFN-γ inhibited the proliferation of pre-osteoclast in a dose-dependent manner (P< 0.05). The apoptosis rate of combined effect of IL-17 and IFN-γwas higher than that of IL-17 alone, and the expression of apoptosis-related gene FasL was significantly higher in IFN-γ with IL-17 than IFN-γ only. Conclusion During the differentiation of RAW264.7 into osteoclasts, IL-17 combined with IFN-γ can inhibit the proliferation of osteoclast precursor cell and promote the apoptosis of RAW264.7.

Key words: Interleukin-17, Interferon-γ, Osteoclast precursor cell, Apoptosis

CLC Number:

R329.2+8

References

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Effect of IL-17 combined with IFN-gamma on the proliferation and apoptosis of RAW264.7 in vitro

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