转化生长因子β3促进兔牙髓干细胞成骨向分化机制的初步研究

Abstract

Abstract: Abstract：0bjective To investigate the effect and the mechanism of TGF-β3 on the osteogenic differentiation of rabbit dental pulp stem cells(rDPSCs).Methods rDPSCs in third passage were divided into the test group(rDPSCs+80ng/ml TGF-β3),control group (rDPSCs+osteogenic medium)and blank group(rDPSCs)；For each group after 7,14d, ALP activity was measured, RT—qPCR was carried out to measure the expression of COL-1A1,Runx-2,OPN,BSP and Smad4,and alizarin red S staining(ARS) was held after14,21 day to testify mineralized matrix productivity. Results ALP activity showed significant difference in each two groups（P＜0.05）. Experimental group showed higher expression of COL-1A1,Runx-2,OPN,BSP and Smad4 than the blank group（P＜0.05） and higher expression of COL-1A1 and Smad4 than the control group（P＜0.05）. Conclusion TGF-β3 can improve the osteogenic differentiation of rDPSCs and this process maybe through activating Smad pathway.

Key words: transforming growth factor-β3（TGF-β3）, rabbit dental pulp stem cells(rDPSCs), osteogenic differentiation, Smad pathway

Muhetaer Huojia. Preliminary study on the mechanism of TGF-β3 in promoting osteogenic differentiation of rabbit dental pulp stem cells[J]. , 2019, 39(2): 97-102.

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Preliminary study on the mechanism of TGF-β3 in promoting osteogenic differentiation of rabbit dental pulp stem cells

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