成釉细胞瘤细胞抑制骨髓间充质干细胞成骨分化的研究

Abstract

Abstract: Abstract: Object: To explore the mechanism of ameloblastoma in inhibiting proliferation, osteogenic differentiation and autophagy level of human bone marrow derived mesenchymal stem cells (hBMSCs) to affect bone regeneration. Method: Human ameloblastoma cells (hAMCs) were isolated and identified. The conditional media was collected to culture the hBMSCs. CCK-8 kit was used to detect the hBMSC proliferation, while ALP staining, Alizarin Red staining and Western-blot were applied to investigate the osteogenic differentiation and autophagy status of the hBMSCs. Results: The osteogenic phenotype of hBMSCs were inhibited by hAMC-CM. The markers of osteogenesis and autophagy in hBMSCs were down-regulated, such as Runx2, Osx, ALP, OCN, OPN, Collagen I, ATG5, Beclin and LC3b. Conclusion: hAMCs could affect autophagy status of hBMSCs via their paracrine functions to inhibit osteogenesis.

Key words: hAMCs, bone marrow mesenchymal stem cells, bone regeneration, autophagy

CLC Number:

R349.5

References 1.

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Research on human amelobastoma cells inhibiting the osteogenic differentiation of hBMSCs

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