Abstract: Abstract：　Objective　To study the effects of Qingpi extract on the growth of six major cariogenic bacteria, and to explore the potential anti-caries mechanism of Qingpi based on network pharmacology. Methods　The MIC and MBC of six major cariogenic bacteria were determined by liquid dilution method. Based on TCMSP, the chemical constituents and corresponding targets of Qingpi were obtained. The related targets of caries were obtained through Gene Cards and OMIM database. The chemical constituents of Qingpi were mapped to disease targets to predict caries. Using Cytoscape software to construct a component-target regulatory network and protein-protein interaction network, use the DAVID database to analyze the GO classification enrichment analysis, and use the Kobas database to analyze the target KEGG signal path analysis. Results　The MIC of Qingpi against S. mutans, S. sanguis, S. sobrinus, A. viscosus, A. naeslundii and L. acidophilus was 1, 0.5, 1, 2, 2, 2mg/mL, MBC was 2, 1, 2, 4, 4, 4mg/mL．According to the results of network pharmacology, there are 12 effective components for the treatment of caries and 28 targets. The key active ingredients include neohesperidin, naringenin and nobiletin. The key targets include TNF, CAT, MMP9 et al. Pathway enrichment analysis showed signal pathways such as AGE-RAGE、NF-κB signaling pathway. Conclusions　The extract of Qingpi has an inhibitory effect on the growth of six major cariogenic bacteria, and network pharmacology reveals the pharmacological basis and mechanism of its chemical constituents against caries. It can provide experimental and theoretical basis for the subsequent development of drugs for preventing caries from the Qingpi.

Key words: Citrus reticulate, cariogenic bacteria, network pharmacology, target, mechanism of action