Abstract: ABSTRACT: Objective To investigate the effects of naringin (NRG) on the proliferation and expression of inflammatory factors of human periodontal ligament fibroblast (HPDLF) induced by lipopolysaccharide (LPS). Methods: HPDLF was induced by lipopolysaccharide (LPS) (100 μg?mL-1). The protective effect of NRG on LPS induced HPDLF injury was detected by MTT assay. The effects of NRG on the expression of IL-6, IL-8, IL?1β and other inflammatory factors in LPS-induced HPDLF were detected by ELISA and Western blotting. The effects of NRG on the expression of IL-6, IL-8 and IL?1β in LPS-induced HPDLF were also detected by Real-time PCR. Results: The proliferation of HPDLF was inhibited by LPS (100 μg?mL-1). Compared with the CON group, the survival rate of cells in LPS group was significantly decreased (P<0.05). Different concentrations of NRG (40, 20, 10 μg?mL-1) could significantly inhibit LPS-induced HPDLF injury and reduce the expression of inflammatory factors in cells. (P<0.05). Compared with LSP group, the survival rate of HPDLF in LPS+NRG 40 μg?mL-1 group, LPS+NRG 20 μg?mL-1 group, LPS+NRG 10 μg?mL-1 group was significantly increased, and the expression levels of inflammatory factors such as IL 6, IL-8, IL?1β were significantly decreased, with significant difference (P<0.05). Conclusion: NRG has protective effect on LPS-induced HPDLF injury, and can promote the proliferation of HPDLF cells by inhibiting the expression levels of IL-6, IL-8, IL?1β and other inflammatory factors in LPS-induced HPDLF.

Key words: naringin, lipopolysaccharide, human periodontal ligament fibroblast, IL-6, IL-8, IL?1β