Abstract: Objective　 To study the expression of adiponectin receptor mRNA in the human gingival fibroblasts(HGFs) of healthy subjects and the effect of tumor necrosis factor alpha,（TNF-α）, Porphyromonas gingivalis（P.g.）lipopolysaccharide (LPS) and adiponectin(APN )on the expression of adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2) mRNA in HGFs to investigate the correlation between the factors related to periodontitis and the expression of adiponectin receptors and. Methods　 The HGFs were cultured in vitro by tissue-adherent method, using reverse transcription polymerase chain reaction(RT-PCR) to detect the expression of adiponectin receptors， and the cells were randomly divided into 4 groups, stimulated with TNF-α50ng/ml ,P.g LPS 0.1mg/ml ,APN 1μg/ml, and a blank control (10% FBS medium) respectively ,lasting for 24 hours, then using real time polymerase chain reaction （real-time PCR） to detect the expression of adiponectin receptor (AdipoR1, AdipoR2) mRNA in HGFs at different time points (0, 0.5, 12, 24h). Results　 RT-PCR showed that HGFs expressed both AdipoR1 and AdipoR2, in which AdipoR2 bands had a higher luminance than AdipoR1, and TNF-a decreased AdipoR2 mRNA expression (p<0.05) in HGFs compared with the blank control group, P.g LPS and APN significantly raised the expression of AdipoR1 and AdipoR2 mRNA (p<0.05).Conclusions　 Adiponectin receptors (AdipoR1, AdipoR2) are both expressed in human gingival fibroblasts, and primarily the AdipoR2. In the condition of periodontal inflammation, the expression of adiponectin receptor decreased, therefore the effect of adiponectin decreased, auxiliary APN receptor agonist and other local drug treatment for the control of inflammation is of great significant to prevent aggravating the disease.

Key words: Periodontitis, Adiponectin recepteor, Adiponectin, TNF-α, Porphyromonas gingivalis lipopolysaccharide