Abstract: Objective To investigate the regulation of miRNA which is negatively correlated with TLR2 on TLR2 and BSP in hPDLFs.Methods Find miRNAs which target and have negative correlation with TLR2;The miRNA mRNA level induced by normal and LPS in hPDLFs were detected by Real-time PCR;The blank contol,the negtive control ,the miRNA mimics,miRNA ASO,as well as their counterpart negtive control group were transfected into cultured hPDLFs by Lipofectamine2000,the mRNA and protein expression were detected by Reat-time PCR and Western Blot.Results It was shown that TLR2 mRNA expression was up-regulated(P=0.000),while the miR-144 mRNA level was down-regulated(P=0.000).Up-regulation of miR-144 could decrease the mRNA(P=0.007) and protein expression of TLR2,while the inhibition of miR-144 had the opposite effect(P=0.017).The BSP expression was down-regulated after induced by LPS(P=0.016); Up-regulation of miR-144 could increase the mRNA(P=0.018) and protein expression of BSP,while the inhibition of miR-144 played the opposite role(P=0.021).Conclusion:MiR-144 can inhibit TLR2 and upregulate BSP expression,has a reverse effect with LPS,which can counteract the activation of TLR2 and inhibition of BSP by LPS in hPDLFs.

Key words: miR-144, Toll-like receptor 2, bone sialoprotein, Porphyromonas gingivals, lipopolysaccharide