慢病毒介导cbfα1基因感染对人牙龈成纤维细胞成骨特性的影响

Abstract

Abstract: Abstract：　Objective To study the effects of cbfα1 gene transfection on human gingival fibroblasts(hGFs). Methods To construct Lentivirus expression vector with the target gene cbfα1,and its construction was verified by enzyme digestion and sequencing. 293T cells were used to package the virus and the virus concentration was determined. The cbfα1 gene was transfected into human gingival fibroblasts by lentivirus. The hGFs were divided into three groups: Lentivirus plenti6.3-cbfα1- Ires-EGFP (pl-cbfα1) (experimental group), plenti6.3- Ires-EGFP (pl-E) (empty vector group), and the untransfected normal hGFs group. The expression of cbfα1 gene were determined by fluorescence real-time quantitative PCR and immunohistochemistry. Expressions of osteogenic genes BSP, OPN, ColI and OC in each group were detected by fluorescence real-time quantitative PCR. Results The results showed that the lentivirus with aim gene was able to infect human gingival fibroblasts successfully, and cbfα1 mRNA and protein were synthesized in the gingival fibroblasts. It was also found that the expressions of osteogenic genes BSP, OPN, ColI and OC in experimental group were significantly higher than those in the control groups(P<0.05). Conclusion The exogenous gene cbfα1 can be expressed in human gingival fibroblasts and promote its transformation to osteoblasts. This study was laying a foundation for further research on the role of this gene and seed cells in periodontal tissue regeneration engineering.

Key words: cbfα1, lentivirus, hGFs

CLC Number:

R349.64

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