VEGF和PDGF联合诱导的骨髓间充质干细胞膜片复合马鹿角粉/PVA支架的体内成血管相关研究

Abstract

Abstract: Objective To explore SD rat bone marrow mesenchymal stem cells (BMSCs) induced by combined application of vascular endothelial growth factor (VEGF) and plateletderived growth factorBB (PDGFBB); to explore its feasibility in vivo and the expression of related angiogenesis, after bone marrow mesenchymal stem cells cell membranered antler powder/PVA scaffold is replanted in animals. Methods Thirtysix 10weekold male SD rats were selected and their weight was controlled at (250±30)g, and they were randomly divided into 3 groups, each with 12 rats. The 3D printed red deer antler powder/PVA scaffold was replanted into the rat kidney capsule, and then gross observation, HE staining, blood vessel count and RTPCR were performed. The animals were classified into 3 groups, experimental group:growth factor induced BMSCs patchstent complex; control group:BMSCs patchstent complex; blank group:no materials were transplanted after modeling. Results After replanting, the expression of factors related to angiogenesis, hypoxia inducible factor1α (hypoxia inducible factor1α, HIF1α), angiopoietin1 (angiopoietin1, Ang1), hepatocyte growth factor (HGF) and insulinlike growth factor1 (IGF1), were all increased (P＜0.05) in experimental group. The HE staining showed obvious angiogenesis in the experimental group. The number of blood vessels in the experimental group was higher than that in the control group. Conclusion The BMSCs membranered antler powder scaffold induced by VEGF and PDGFBB can effectively promote the formation of new blood vessels and contribute to the vascularization of tissue engineered bone.

Key words: VEGF, PDGF, angiogenesis, cell membrane, tissue engineering

CLC Number:

R783.1

References 20

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In vivo study of vascularization of bone marrow mesenchymal stem cell membrane combined with red antler powder/PVA scaffold induced by VEGF and PDGF

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