双硫仑联合铜通过调控视神经萎缩蛋白1诱导舌癌细胞凋亡

Abstract

Abstract: Objective　To investigate the effect of disulfiram combination with cooper (DSF-Cu) on the apoptosis of Cal-27 cells and its relation with optic atrophy 1 (OPA1). Methods　The Cal-27 cells were treated with various concentrations of DSF-Cu. Cell viability was evaluated by MTT assays. The apoptosis rate was analyzed by flow cytometry. The protein expression level of OPA1 was detected by western blotting. Results As compared with control group, DSF-Cu decreased the number of viable cells．Flow cytometric analysis showed an increase in the incidence of apoptosis. What’s more, the content of ATP was decreased significantly. Western blot results showed the expression of OPA1 was markedly decreased in high and medium concentration group, but no significant change in low concentration group. Conclusions　DSF-Cu can induce apoptosis of Cal-27 cells, which may be achieved by inhibiting the expression of OPA1.

Key words: disulfiram, tongue carcinoma cell, OPA1, apoptosis

CLC Number:

R739.8

References 20

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Disulfiram combination with cooper induces apoptosis of tongue carcinoma cells by regulating OPA1 expression

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