DHA对人牙龈成纤维细胞生物学活性及炎症因子表达的作用

Abstract

Abstract: ObjectiveTo explore the effects of docosahexaenoic acid (DHA) on the biological activity and inflammatory response of human gingival fibroblasts (HGFs).MethodsThe effects of DHA on cell viability, cell morphology and cell cycle were observed by living and dead cell staining, fluorescent staining and flow cytometry, respectively. After pretreated with DHA, HGFs were stimulated with lipopolysaccharide (LPS) from Porphyromonasgingivalis (P. gingivalis) or with heat- inactivated P. gingivalis. Then the gene and protein expression of interleukin-6 (IL-6), IL-8 and IL-1β was detected by quantitative PCR and ELISA, respectively. The effect of DHA on heme oxygenase-1 (HO-1) gene and protein expression was also observed by quantitative PCR and western blot, respectively. Results200 μmol/L DHA reduced the cell activity of HGFs, but 100 μmol/L DHA had no effect on cell viability and morphology, and had no significant effect on cell cycle (P>0.05); DHA inhibited the expression of IL-6, IL-8, IL-1β mRNA and IL-6, IL-8 protein induced by P. gingivalis LPS or heat- inactivated P. gingivalis in a concentration-dependent manner (P<0.05); DHA significantly induced HO-1 mRNA and protein expression (P<0.05).ConclusionDHA significantly inhibited the inflammatory response of HGFs without affecting the biological activity. The anti-inflammatory effect may be related to the induction of HO-1.

Key words: docosahexaenoic acid, human gingival fibroblasts, biological activity, inflammatory response, heme oxygenase-1

CLC Number:

R781.4

References 16

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Effects of DHA on biological activity and inflammatory factor expression of human gingival fibroblasts

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