Abstract: Objective To investigate the effects of Asperosaponin Ⅵ on osteogenic differentiation of mouse myoblasts. Methods The concentrations of Asperosaponin Ⅵ were 1×10-3–1×10-8 mol/L. Cell counting kit 8 was used to detect the cytotoxicity and proliferation ability of Asperosaponin Ⅵ on mouse myoblasts. The optimal concentration of ALP was determined by alkaline phosphatase activity analysis. Finally, Western blot analysis, real-time quantitative PCR and alizarin red staining were used to observe the effect of osteogenic differentiation. Results The results showed that 1×10-3、1×10-4 mol/L Asperosaponin Ⅵ had obvious toxic effects on mouse myoblasts (P<0.01), while 1×10-5–1×10-8mol/L had no obvious effect on cell proliferation. It was also shown that 1×10-6mol/L Asperosaponin Ⅵ significantly promoted the osteogenic differentiation of mouse myoblasts (P<0.01). Compared with the control group, the mRNA and protein expressions of ALP, Runx2, Ocn and col1 α 1 in the concentration of 1×10-6 mol/L Asperosaponin Ⅵ were significantly increased (P<0.05), and alizarin red staining also showed more calcium nodules. Conclusion 1×10-6mol/L Asperosaponin Ⅵ can significantly promote the osteogenic differentiation of mouse myoblasts, but has no obvious effect on its proliferation.

Key words: Asperosaponin VI, mouse myoblasts, osteogenic differentiation