低水平激光通过线粒体自噬通路促进正畸牙移动的作用研究

doi:10.13591/j.cnki.kqyx.2026.03.004

Abstract

Abstract:

Objective To investigate the effect of low-level laser therapy (LLLT) on orthodontic tooth movement (OTM) in rats and its underlying mechanism. Methods An OTM rat model, which was combined with LLLT, was established. The mesial movement distance of the maxillary first molars was compared between the force-applied with LLLT group (F⁺L⁺ group) and the force-applied only group (F⁺L^- group) using micro-CT analysis. A cellular stress model was constructed using THP-1 cells subjected to compressive force and laser irradiation. mRNA sequencing was performed to identify differentially expressed genes (DEGs) between force-applied with laser irradiation THP-1 cells and force-applied only cells, followed by functional enrichment analysis. The expression changes of pathway-related genes and markers were further validated by quantitative real-time PCR (qRT-PCR) and Western blot. Results The mesial movement distance of the maxillary first molars was significantly greater in the F⁺L⁺ group compared to the F⁺L^- group (P<0.05). DEG analysis revealed significant enrichment in the mitophagy pathway in the F⁺L⁺ THP-1 cells versus the F⁺L^- cells. qRT-PCR and Western blot confirmed that the expression levels of the mitophagy-related genes OPTN and SQSTM1, whose products are key adaptors in the process, were decreased in the F⁺L⁺ cells. Concurrently, the key mitophagy initiators PINK1 and Parkin were also downregulated. Conclusion LLLT effectively accelerates OTM in rats. This acceleratory effect may be mediated through the inhibition of the mitophagy pathway.

Key words: low-level laser therapy (LLLT), orthodontics, tooth movement, mitophagy

CLC Number:

R783.5

WANG Xiaochen, LIN Junyan, GUO Peipei, ZHANG Rui, LI Dandan. Effect of low-level laser therapy on orthodontic tooth movement via the mitophagy pathway[J]. Stomatology, 2026, 46(3): 181-188.

Figures/Tables 6

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基因名称	引物序列
SQSTM1	F:5'-GACTACGACTTGTGTAGCGTC-3',R:5'-AGTGTCCGTGTTTCACCTTCC-3'
SRC	F:5'-GAGCGGCTCCAGATTGTCAA-3',R:5'-CTGGGGATGTAGCCTGTCTGT-3'
TFEB	F:5'-ACCTGTCCGAGACCTATGGG-3',R:5'-CGTCCAGACGCATAATGTTGTC-3'
GABARAPL1	F:5'-ATGAAGTTCCAGTACAAGGAGGA-3',R:5'-GCTTTTGGAGCCTTCTCTACAAT-3'
HIF1A	F:5'-GAACGTCGAAAAGAAAAGTCTCG-3',R:5'-CCTTATCAAGATGCGAACTCACA-3'
OPTN	F:5'-AAAGAGCGTCTAATGGCCTTG-3',R:5'-GTTCAGACACGATGCCCAACA-3'
RELA	F:5'-ATGTGGAGATCATTGAGCAGC-3',R:5'-CCTGGTCCTGTGTAGCCATT-3'

Effect of low-level laser therapy on orthodontic tooth movement via the mitophagy pathway

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