高碱抗性粪肠球菌通过ZBP1诱导弱化的巨噬细胞泛凋亡

doi:10.13591/j.cnki.kqyx.2026.04.004

Abstract

Abstract:

Objective To investigate how highly alkali-resistant Enterococcus faecalis(HAR E. faecalis) regulates Z-DNA binding protein 1(ZBP1) and macrophage PANoptosis(pyroptosis, apoptosis, necrosis). Methods Highly alkali-resistant Enterococcus faecalis was constructed by continuous alkaline subculturing. RAW264.7 macrophages were infected with either the highly alkali-resistant or standard Enterococcus faecalis(E. faecalis). Flow cytometry, live/dead cell staining, confocal microscopy, and Western blot were employed to analyze ZBP1 expression levels and macrophage death pathways. Small interfering RNA(siRNA) was used to knock down ZBP1 expression in macrophages, followed by the same infection protocol. Western blot was used to analyze changes in the expression of PANoptosis markers after ZBP1 knockdown. Results The highly alkali-resistant Enterococcus faecalis exhibited a weaker ability to upregulate the expression of ZBP1 and PANoptosis markers in macrophages compared to the standard strain. Following ZBP1 knockdown, the expression of these markers decreased, with no significant differences observed between the two strains. Conclusion Highly alkali-resistant Enterococcus faecalis induces compromised macrophage PANoptosis by weak activation of ZBP1, which impairs the host’s ability to completely eradicate the bacteria, thereby possibly favoring the occurrence and development of persistent apical periodontitis.

Key words: Enterococcus faecalis, alkali-resistance, Z-DNA binding protein 1, PANoptosis

CLC Number:

R781.3

XIAO Yifang, LIU Runze, OU Dongchen, FAN Wei. Highly alkali-resistant Enterococcus faecalis induces compromised PANoptosis of macrophage via ZBP1[J]. Stomatology, 2026, 46(4): 260-265.

Figures/Tables 7

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References 31

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基因	引物序列(5'—3')
siZBP1	F:GGACAUAGAAAGCUCUCAATT R:UUGAGAGCUUUCUAUGUCCTT
NC-siRNA	F:UUCUCCGAACGUGUCACGUTT R:ACGUAGCACGUUCGGAGAATT

Highly alkali-resistant Enterococcus faecalis induces compromised PANoptosis of macrophage via ZBP1

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