Abstract: Objective To observe and evaluate the clinical efficacy and safety of collagen-matrix bone materials in repair of periodontal bone defect. Methods Patients with periodontitis were divided into 2 groups in a randomized, double-blind manner. A group received bone grafts with collagen-matrix bone materials. B group received Ruifu nano artificial bone. The probing depth (PD), clinical attachment loss (CAL), sulcus bleeding index (SBI), tooth mobility, and alveolar bone healing status were evaluated before surgery and three, six months after surgery. Results Both collagen-matrix bone and nano artificial bone significantly decreased PD, CAL, SBI, or TM of surgery teeth (P<0.01). There was no statistical difference between A and B groups (P>0.05). Conclusions The application of collagen-matrix bone can improve periodontal indexes. The collagen-matrix bone materials may repair periodontal bone defect effectively and safely in periodontal bone grafts surgery.