慢性间歇性缺氧小鼠下颌骨三维结构及组织形态

doi:10.13591/j.cnki.kqyx.2022.03.002

Abstract

Abstract: Objective To observe the effect of long-term chronic intermittent hypoxia caused by obstructive sleep apnea hypoxia on three-dimensional structure and morphology of mandible. Methods Twelve 8-week-old male C57BL/6 mice were divided into two groups randomly. The CIH group was fed with intermittent hypoxia and the control group was fed with normoxia. Four weeks later, the two groups were killed by being excessively anesthetized and the right mandible was detected using micro CT analysis. Morphological changes of mandibles were detected with Hematoxylin-esoin staining. Results At four weeks after modeling, the micro CT technique was used to observe the three-dimensional structure of mandibles in two groups. The quantitative analysis on the microstructure of cancellous bone showed that bone mass in the region of interest in CIH mice were significantly decreased when compared to the control group (P<0.05). Hematoxylin-esoin staining showed that the number and volume of mandibular trabecular of CIH mice were decreased. The results suggested that three-dimensional structure and microstructure of the cancellous bone in CIH mince changed significantly. Conclusion Chronic intermittent hypoxia inhibits bone formation and affects bone metabolism.

Key words: obstructive sleep apnea hypoxia, chronic intermittent hypoxia, mandible, micro CT

CLC Number:

R783.5

GU Hong, WANG Wei, ZHANG Weibing. Three-dimensional structure and morphology of mandible in chronic intermittent hypoxia mice[J]. Stomatology, 2022, 42(3): 200-203.

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Three-dimensional structure and morphology of mandible in chronic intermittent hypoxia mice

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