Stomatology ›› 2024, Vol. 44 ›› Issue (6): 408-413.doi: 10.13591/j.cnki.kqyx.2024.06.002

• Basic and Clinical Research • Previous Articles     Next Articles

Effect of chloroquine on Candida albicans biofilms and its drug resistance

WU Qiaochu,SHI Banruo,MIAO Haochen,WEI Xin()   

  1. Department of Conservative Dentistry and Endodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases (Nanjing Medical University), Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China
  • Received:2024-01-05 Online:2024-06-28 Published:2024-06-27

Abstract:

Objective To evaluate the effects of chloroquine alone and in combination with traditional antifungal agents on the Candida albicans biofilms and its drug resistance. Methods This study used standard strains of Candida albicans, and drug-resistant strains of Candida albicans. The inhibitory effects of chloroquine alone and in combination with antifungal drugs on biofilms of Candida albicans were detected by XTT reduction method and chessboard dilution method respectively. The morphological characteristics of biofilms were observed under scanning electron microscopy. Results Chloroquine at the concentration of 50 μmol/L or above showed a direct inhibitory effect and increased with concentration. Chloroquine combined with amphotericin B had a synergistic inhibitory effect. Results of the time-killing curve showed that the growth trends of biofilms treated with chloroquine alone and combined with amphotericin B varied in different time periods during the experimental culture. Morphological observation also revealed that chloroquine alone and in combination with amphotericin B could reduce the ability of Candida albicans to form hyphae and biofilms. Conclusion Chloroquine has an inhibitory effect on Candida albicans biofilms and can reduce its drug resistance. Furthermore, chloroquine shows a synergistic anti-fungal effect when combined with amphotericin B.

Key words: Candida albicans, antifungal agent, chloroquine

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