ROS响应型纳米载体用于牙髓炎症的抗氧化治疗

doi:10.13591/j.cnki.kqyx.2025.07.006

Abstract

Abstract:

Objective To explore the effects of cerium-doped mesoporous bioactive glass nanoparticles (Ce-MBGNs) as reactive oxygen species (ROS)-responsive nanocarriers on inflammation in pulp and their potential to promote odontogenic differentiation of dental pulp stem cells (DPSCs). Methods The cytotoxicity of Ce-MBGNs was assessed by CCK-8. The expression of mRNA of osteogenic/odontogenic differentiation in DPSCs and that of inflammatory factor in RAW264.7 cells were detected by RT-qPCR. Alkaline phosphatase and Alizarin Red S staining were utilized to investigate their ability to promote dentin mineralization. The ability of Ce-MBGNs to scavenge ROS was evaluated by immunofluorescence and JC-1 staining was used to assess the membrane potential (MMP) of mitochondrial. Results Ce-MBGNs exhibited good biocompatibility and significantly increased the expression of osteogenic/odontogenic-related genes in DPSCs, promoting mineralization. Additionally, Ce-MBGNs effectively scavenged intracellular ROS, maintained MMP, inhibited the expression of pro-inflammatory factors, and promoted the expression of anti-inflammatory factors. Conclusion Ce-MBGNs can protect DPSCs from oxidative damage by maintaining the MMP of mitochondrial and controlling inflammation, and promote their odontoblastic differentiation, providing a theoretical basis for the development of novel therapeutic agents for oral treatment.

Key words: Ce-MBGNs, dental pulp stem cells, immunomodulation, osteogenic/odontoblastic differentiation

CLC Number:

R780.2

WANG Mingxin, DUAN Yiyuan, FENG Kejia, NIE Chenyu, MA Qian. A novel ROS-responsive nanocarrier for antioxidant therapy in endodontic inflammation[J]. Stomatology, 2025, 45(7): 511-517.

Figures/Tables 7

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References 28

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基因名称	引物序列
ALP	Forward:5'-AACATCAGGGACATTGACGTG-3' Reverse:5'-GTATCTCGGTTTGAAGCTCTTCC-3'
DSPP	Forward:5'-GGGAATAGAAATCAAGGGTC-3' Reverse:5'-CAAGATCATTCCATGTTGTCC-3'
DMP-1	Forward:5'-GGTATCACACCCAACTATGAAGATCA-3' Reverse:5'-CCATCATACTGAGCAGCAAAGTTC-3'
BMP2	Forward:5'-ACCCGCTGTCTTCTAGCGT-3' Reverse:5'-TTTCAGGCCGAACATGCTGAG-3'
GAPDH	Forward:5'-CAAGGTCATCCATGACAACTTTG-3' Reverse:5'-GTCCACCACCCTGTTGCTGTAG-3'

基因名称	引物序列
IL-6	Forward:5'-CTGCAAGAGACTTCCATCCAG-3' Reverse:5'-AGTGGTATAGACAGGTCTGTTGG-3'
IL-10	Forward:5'-TCAAGGCGCATGTGAACTCC-3' Reverse:5'-GATGTCAAACTCACTCATGGCT-3'
GAPDH	Forward:5'-CCGAGACCAACCGAGTCATTTA-3' Reverse:5'- GATGGTGATGGGTTTCCCGT-3'

A novel ROS-responsive nanocarrier for antioxidant therapy in endodontic inflammation

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