白藜芦醇和环糊精包合物合成及再矿化作用评价

doi:10.13591/j.cnki.kqyx.2023.12.002

摘要/Abstract

摘要：

目的制备并鉴定白藜芦醇和环糊精包合物,分析包合物对牙本质再矿化的影响。方法以环糊精、白藜芦醇为原料,采用冷冻干燥法制备白藜芦醇-环糊精包合物,采用FTIR、TG对包合物结构进行表征。通过CCK8比较白藜芦醇和包合物的细胞毒性。SEM分析包合物对牙本质再矿化的影响。结果 FTIR图示,与游离白藜芦醇相比,包合物出现了不同频带信号强度降低及消失。热重结果示,白藜芦醇在345 ℃时迅速发生质量损失,环糊精-白藜芦醇包合物组在342 ℃时迅速发生重量损失。这些表征证明白藜芦醇被成功包含在环糊精内。CCK8结果示白藜芦醇在浓度为5 μg/mL时已经表现出明显细胞毒性,包合物组在10 μg/mL时才表现出明显细胞毒性。白藜芦醇被环糊精包合后,细胞毒性降低。SEM图显示,对比纯酸蚀组,白藜芦醇及两种包合物处理后,牙本质脱矿处均出现矿物质生成,包合物组牙本质矿物质生成更为明显。结论环糊精能够成功包合白藜芦醇形成包合物,包合物能够促进牙本质再矿化。

关键词: 再矿化, 白藜芦醇, 包合物

Abstract:

Objective To prepare and identify the inclusion complex of resveratrol and cyclodextrin, and analyze the impact of the complex on dentin remineralization. Methods Resveratrol and cyclodextrin were used as raw materials to prepare the resveratrol-cyclodextrin inclusion complex using freeze-drying method. The structures of the inclusion complex were characterized by FTIR and TG. Cell cytotoxicity of resveratrol and the inclusion complex was compared using CCK8 assay. SEM was used to analyze the effect of the inclusion complex on dentin remineralization. Results FTIR spectra showed that compared to resveratrol, the inclusion complexes exhibited decreased and disappeared signal intensities in different frequency bands. Thermal analysis results demonstrated that resveratrol rapidly lost weight at 345 ℃, while the cyclodextrin-resveratrol inclusion complex exhibited rapid weight loss at 342 ℃. The characterization confirmed the successful encapsulation of resveratrol within cyclodextrin. CCK8 results indicated that resveratrol exhibited significant cytotoxicity at a concentration of 5 μg/mL, while the complex showed significant cytotoxicity at 10 μg/mL. The cytotoxicity of resveratrol was reduced after inclusion with cyclodextrin. SEM images indicated that, compared to the pure acid-etched group, both resveratrol and inclusion complexes resulted in mineral formation at demineralized dentin areas, with the group of inclusion complex showing more pronounced dentin remineralization. Conclusion Cyclodextrincan successfully form inclusion complexes with resveratrol, and the complexes promote dentin remineralization.

Key words: remineralization, resveratrol, inclusion complex

中图分类号:

R783.1

黄路梅,陈晨. 白藜芦醇和环糊精包合物合成及再矿化作用评价[J]. 口腔医学, 2023, 43(12): 1065-1069.

HUANG Lumei,CHEN Chen. Synthesis and remineralization evaluation of resveratrol and cyclodextrin inclusion complex[J]. Stomatology, 2023, 43(12): 1065-1069.

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