慢性间歇性缺氧小鼠下颌骨三维结构及组织形态

doi:10.13591/j.cnki.kqyx.2022.03.002

摘要/Abstract

摘要： 目的探讨阻塞性睡眠呼吸暂停(obstructive sleep apnea hypoxia,OSAH)引起的长期慢性间歇性低氧(CIH)对下颌骨三维结构以及组织形态的影响。方法 12 只8 周C57BL/6雄性小鼠随机分成两组,CIH组以间歇性缺氧饲养,对照组(CON)以常氧饲养,4 周后麻醉处死小鼠取右侧下颌骨行micro CT检测观察其三维结构,苏木精-伊红染色观察下颌骨组织学变化。结果造模4周后,micro CT观察两组小鼠的下颌骨三维结构,对其松质骨含量进行定量分析显示,缺氧组小鼠下颌骨感兴趣区域松质骨量比对照组小鼠显著减少(P<0.05)。苏木精-伊红染色显示,缺氧组小鼠下颌骨松质骨骨小梁数量和体积均变小,说明慢性间歇性缺氧小鼠下颌骨松质骨三维结构和骨组织微结构均发生显著改变。结论慢性间歇性低氧抑制骨形成影响骨代谢。

关键词: 阻塞性睡眠呼吸暂停, 慢性间歇性缺氧, 下颌骨, micro CT

Abstract: Objective To observe the effect of long-term chronic intermittent hypoxia caused by obstructive sleep apnea hypoxia on three-dimensional structure and morphology of mandible. Methods Twelve 8-week-old male C57BL/6 mice were divided into two groups randomly. The CIH group was fed with intermittent hypoxia and the control group was fed with normoxia. Four weeks later, the two groups were killed by being excessively anesthetized and the right mandible was detected using micro CT analysis. Morphological changes of mandibles were detected with Hematoxylin-esoin staining. Results At four weeks after modeling, the micro CT technique was used to observe the three-dimensional structure of mandibles in two groups. The quantitative analysis on the microstructure of cancellous bone showed that bone mass in the region of interest in CIH mice were significantly decreased when compared to the control group (P<0.05). Hematoxylin-esoin staining showed that the number and volume of mandibular trabecular of CIH mice were decreased. The results suggested that three-dimensional structure and microstructure of the cancellous bone in CIH mince changed significantly. Conclusion Chronic intermittent hypoxia inhibits bone formation and affects bone metabolism.

Key words: obstructive sleep apnea hypoxia, chronic intermittent hypoxia, mandible, micro CT

中图分类号:

R783.5

顾红, 王威, 张卫兵. 慢性间歇性缺氧小鼠下颌骨三维结构及组织形态[J]. 口腔医学, 2022, 42(3): 200-203.

GU Hong, WANG Wei, ZHANG Weibing. Three-dimensional structure and morphology of mandible in chronic intermittent hypoxia mice[J]. Stomatology, 2022, 42(3): 200-203.

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