熊果苷通过NF-κB信号通路减轻脂多糖对人牙周膜干细胞成骨分化的抑制作用

doi:10.13591/j.cnki.kqyx.2025.05.005

摘要/Abstract

摘要：

目的探究熊果苷对炎症状态下人牙周膜干细胞成骨分化的影响及NF-κB信号通路在其中的作用机制。方法通过CCK-8、碱性磷酸酶染色、茜素红染色及Western blot分析熊果苷对人牙周膜干细胞增殖及成骨分化的影响。利用脂多糖刺激人牙周膜干细胞建立体外炎症模型后,通过RT-qPCR分析熊果苷对炎症因子表达水平的影响,通过前述方法分析熊果苷对细胞成骨分化能力的影响,通过Western blot分析熊果苷对NF-κB通路的影响。向培养体系加入NF-κB信号通路激活剂佛波酯后,分析熊果苷的作用是否改变。结果 100 nmol/L 熊果苷不影响人牙周膜干细胞的增殖,显著促进成骨分化,抑制脂多糖刺激下炎症因子的表达。熊果苷减轻了脂多糖对NF-κB信号通路的激活作用及对细胞成骨分化的抑制作用,而熊果苷的功效被佛波酯部分消除。结论熊果苷通过抑制NF-κB信号减轻脂多糖对人牙周膜干细胞成骨分化的抑制作用。

关键词: 牙周膜干细胞, 熊果苷, 牙周炎, 成骨, NF-κB

Abstract:

Objective To investigate the effect of arbutin (Arb) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions and the mechanism of NF-κB signaling pathway in this process. Methods The effects of Arb on the proliferation and osteogenic differentiation of hPDLSCs were analyzed by CCK-8, alkaline phosphatase staining, Alizarin Red staining, and Western blot. After establishing an inflammatory model using lipopolysaccharide (LPS), the effect of Arb on the expression levels of inflammatory factors in hPDLSCs was analyzed by RT-qPCR. The effect of Arb on osteogenic differentiation of hPDLSCs was analyzed, and the effect of Arb on the NF-κB pathway was analyzed by Western blot. After adding the NF-κB signaling pathway activator PMA to the culture system, whether the effect of Arb on hPDLSCs changed was analyzed. Results 100 nmol/L Arb did not affect the proliferation of hPDLSCs, but significantly promoted cell osteogenic differentiation and inhibited the expression of inflammatory factors under LPS stimulation. Arb reduced the activation effect of LPS on the NF-κB signaling pathway and the inhibitory effect on cell osteogenic differentiation, while the efficacy of Arb was partially eliminated by PMA. Conclusion Arb alleviates the inhibitory effect of LPS on osteogenic differentiation of hPDLSCs by inhibiting NF-κB signaling.

Key words: periodontal ligament stem cells, arbutin, periodontitis, osteogenesis, NF-κB

中图分类号:

R783

高晶萍, 贾凌璐, 梁洪宁, 文勇. 熊果苷通过NF-κB信号通路减轻脂多糖对人牙周膜干细胞成骨分化的抑制作用[J]. 口腔医学, 2025, 45(5): 347-354.

GAO Jingping, JIA Linglu, LIANG Hongning, WEN Yong. Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway[J]. Stomatology, 2025, 45(5): 347-354.

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引物	Forward primer 5'-3'	Reverse primer 5'-3'
IL-6	AGCCCACCGGGAACGA	GGACCGAAGGCGCTTGT
IL-8	TTGGCAGCCTTCCTGATTTC	AACTTCTCCACAACCCTCTGCA
CCL2	TGACCCCAAGAAGGAATGGG	ACCTTAGGGCAGATGCAGTT
GAPDH	AGAAGGCTGGGGCTCA7ITITIG	AGGGGCCATCCACAGTCTTC