口腔医学 ›› 2025, Vol. 45 ›› Issue (6): 418-423.doi: 10.13591/j.cnki.kqyx.2025.06.004

• 基础与临床研究 • 上一篇    下一篇

miR-129-1-3p通过BMP2/SMAD1信号通路抑制人骨髓间充质干细胞成骨分化的研究

耿铭珠1,2,3, 穆文清1,2,3, 邱琳1,2,3, 张玮1,2,3()   

  1. 1 南京医科大学附属口腔医院口腔特诊科,江苏南京(210029)
    2 口腔疾病研究与防治国家级重点实验室培育建设点,江苏南京(210029)
    3 江苏省口腔转化医学工程研究中心,江苏南京(210029)
  • 收稿日期:2025-01-22 出版日期:2025-06-28 发布日期:2025-07-08
  • 通讯作者: 张玮 E-mail: zhangwei0508@njmu.edu.cn
  • 基金资助:
    江苏省自然科学基金青年科技人才专项基金(BK20171057);江苏省科教能力提升工程——江苏省研究型医院建设单位(YJXYYJSDW4);江苏省医学创新中心(CXZX202227)

miR-129-1-3p inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells via BMP2/SMAD1 signaling pathway

GENG Mingzhu1,2,3, MU Wenqing1,2,3, QIU Lin1,2,3, ZHANG Wei1,2,3()   

  1. Department of Oral Special Consultation, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2025-01-22 Online:2025-06-28 Published:2025-07-08

摘要:

目的 探讨miR-129-1-3p对人骨髓间充质干细胞(human bone marrow mesenchymal stem cells,hBMSCs)成骨分化的作用及相关机制研究。方法 构建空白对照(NC组)、miR-129-1-3p模拟物(Mimic组)、miR-129-1-3p抑制物(Inhibitor组)及其相应的阴性对照组(Mimic-NC组、Inhibitor-NC组)并转染至hBMSCs。碱性磷酸酶和茜素红染色观察钙盐矿化结节形成能力,qRT-PCR检测miR-129-1-3p及成骨分化标志物表达水平,western blot检测骨形态发生蛋白2(bone morphogenetic protein 2,BMP2)、SMAD1及磷酸化SMAD1蛋白表达水平。结果 转染后,相较于Mimic-NC组,Mimic组miR-129-1-3p表达水平显著升高(P<0.05),矿化结节显著减少,BMP2、Runt相关转录因子2(Runt-related transcription factor 2,RUNX2)、骨钙素(osteocalcin,OCN)mRNA表达水平显著下调(P<0.05),BMP2和磷酸化SMAD1蛋白表达水平显著下调(P<0.05)。Inhibitor组相较于Inhibitor-NC组矿化结节明显增多,BMP2、RUNX2、OCN mRNA表达显著上调(P<0.05),BMP2和磷酸化SMAD1蛋白表达水平显著上调(P<0.05)。结论 miR-129-1-3p可能通过调控BMP2/SMAD1信号通路抑制人骨髓间充质干细胞成骨分化。

关键词: miR-129-1-3p, BMP2/SMAD1信号通路, 人骨髓间充质干细胞, 成骨分化, 骨质疏松

Abstract:

Objective To investigate the effect of miR-129-1-3p on the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and its potential mechanism. Methods Negative control group, the miR-129-1-3p mimic group, the miR-129-1-3p inhibitor group and the corresponding negative control were constructed and transfected into hBMSCs. The formation of calcium-mineralized nodules was observed by alkaline phosphatase staining and alizarin red S staining. The expression levels of miR-129-1-3p and osteogenic differentiation markers were detected by qRT-PCR. Western blot detected the protein expressions of bone morphogenetic protein 2 (BMP2), SMAD1 and p-SMAD1. Results After transfection, the expression level of miR-129-1-3p in mimic group was significantly increased (P<0.05), the number of mineralized nodules was significantly decreased, and the expression levels of BMP2, Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN) mRNA were significantly down-regulated (P<0.05). BMP2 and p-SMAD1 protein were also significantly down-regulated (P<0.05) compared with Mimic-NC group. The expression levels of BMP2, RUNX2, OCN mRNA were significantly up-regulated in inhibitor group (P<0.05) compared with Inhibitor-NC group. BMP2 and p-SMAD1 protein were significantly up-regulated in inhibitor group (P<0.05). Conclusion miR-129-1-3p can inhibit the osteogenic differentiation of human bone marrow mesenchymal stem cells by suppressing BMP2/SMAD1 signaling pathway.

Key words: miR-129-1-3p, BMP2/SMAD1 signaling pathway, human bone marrow mesenchymal stem cells, osteogenic differentiation, osteoporosis

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