牙龈卟啉单胞菌脂多糖对THP-1源性巨噬细胞CD36和LOX-1表达的影响

›› 2015, Vol. 35 ›› Issue (8): 614-618.

牙龈卟啉单胞菌脂多糖对THP-1源性巨噬细胞CD36和LOX-1表达的影响

岑胜丹¹,陈利娇¹,胡招玲²,邓辉¹,胡荣党³

1. 温州医科大学附属口腔医院
2. 温州医科大学口腔医学院
3. 温州医科大学附属口腔医院牙周科

收稿日期:2014-11-10 修回日期:2015-05-21 出版日期:2015-08-28 发布日期:2015-08-14
通讯作者: 邓辉 E-mail:dh0726@163.com
基金资助:
国家自然科学基金;浙江省自然科学基金;2013年浙江省大学生科技创新活动计划（新苗人才计划）立项资助项目

The effect of porphyromonas gingivalis lipopolysaccharides on the expression of CD36 and LOX-1 macrophages derived from THP-

Received:2014-11-10 Revised:2015-05-21 Online:2015-08-28 Published:2015-08-14

摘要/Abstract

摘要： 目的该文以THP-1源性巨噬细胞为研究对象，研究牙龈卟啉单胞菌脂多糖（P.g-LPS）对巨噬细胞内胆固醇代谢关键分子CD36、LOX-1表达的影响。方法以160 nmol/L佛波酯（PMA）诱导THP-1单核细胞48 h，使其分化为巨噬细胞。建立P.g-LPS与THP-1源性巨噬细胞共孵育的体外模型，在负荷50 μg/mL低密度脂蛋白（LDL）的条件下，分别用浓度为0.1、1.0、10.0 μg/mL P.g-LPS与巨噬细胞共孵育48 h；1 μg/mL P.g-LPS与巨噬细胞共孵育24、48和72 h。采用实时荧光定量PCR和Western blot方法探讨巨噬细胞胆固醇代谢关键分子CD36和LOX-1表达变化。结果与空白对照组相比，随着P.g-LPS浓度的增加和1 μg/mL P.g-LPS孵育时间的延长，巨噬细胞内CD36 mRNA和蛋白表达均逐渐下降；LOX-1mRNA和蛋白表达无明显变化。结论 P.g-LPS可促进巨噬细胞CD36的表达，对LOX-1表达无影响，从而可能对巨噬细胞脂质代谢产生影响。

关键词: 牙龈卟啉单胞菌, 脂多糖, THP-1单核细胞, CD36, LOX-1

Abstract: Objective To explore the effect of porphyromonas gingivalis lipopolysaccharides (P.gLPS) on the expression of CD36 and LOX1 macrophages derived from THP1.Methods THP1 cells were cultured in the presence of 160 nmol/L phorbol 12myristate 13acetate (PMA) for 48 h.An cocultured in vitro model of P.gLPS and THP1 macrophage cells was constructed.In the presence of LDL (50 μg/ml),macrophage cells were incubated with P.gLPS (0.1,1,10 μg /ml) for 48 h or with 1μg/ml P.gLPS for 24,48,72h.The expression of CD36,LOX1 at mRNA and protein levels was determined by real time PCR and Western blot.Results THP1 cells were differentiated into macrophages after the addition of PMA for 48h.P.gLPS downregulated the mRNA and protein expression of CD36 as concentration increased and time extended in the presence of LDL (P<0.05).However,P.gLPS had no effect on the expression of LOX1 at mRNA and protein levels compared with control group.Conclusions P.gLPS downregulated CD36,but had no effect on the expression of LOX1,which may contribute to the excessive accumulation of cholesteryl esters and conversion to foam cells in macrophages.

Key words: Porphyromonas gingivalis, lipopolysaccharide, THP-1 monocytes, CD36, LOX-1

中图分类号:

R780.2

岑胜丹陈利娇胡招玲邓辉胡荣党. 牙龈卟啉单胞菌脂多糖对THP-1源性巨噬细胞CD36和LOX-1表达的影响[J]. 口腔医学, 2015, 35(8): 614-618.

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