应用iRoot BP Plus、MTA直接盖髓后牙髓修复反应变化的大鼠实验研究

doi:10.13591/j.cnki.kqyx.2025.03.006

摘要/Abstract

摘要：

目的探讨iRoot BP Plus、MTA直接盖髓术后牙髓修复反应变化的异同。方法将36只大鼠随机分为3组,即iRoot BP Plus组、MTA组及空白对照组。对每只大鼠上颌左右第一磨牙进行开髓并行直接盖髓术。术后 7、14、28 d于每组中随机选择4只大鼠处死、取标本,行苏木精-伊红(hematoxylin-eosin staing,HE)染色,并观察各指标。结果 ①从牙本质小管形成质量来看,14、28 d时,iRoot BP Plus 组与MTA组比较均具有统计学差异(P<0.05),且iRoot BP Plus 在修复性牙本质形成质量方面明显优于MTA。②从修复性牙本质新生面积比值来看,术后14 d,iRoot BP Plus组较MTA组高(P<0.05)。结论 iRoot BP Plus、MTA直接盖髓术后均可促进修复性牙本质桥的形成,且形成质量较高,远期效果有待进一步研究。

关键词: iRoot BP Plus, MTA, 直接盖髓术, 牙髓修复性牙本质

Abstract:

Objective To explore the differences and similarities in the changes of pulp repair response after direct pulp capping with iRoot BP Plus and MTA. Methods A total of 36 male SD rats were randomly divided into 3 groups, namely iRoot BP Plus group, MTA group and blank control group. Dental pulp exposure and direct pulp capping was performed on both sides of maxillary first molars of each rat. Four rats were randomly selected and sacrificed from each group at 7, 14, and 28 days postoperatively, and the specimens were taken for hematoxylin-eosin (HE) staining, and then each index was observed. Results ①From the perspective of dentin tubular formation quality, at 14 d and 28 d, there were statistical differences between iRoot BP Plus group and MTA group(P<0.05), and iRoot BP Plus was significantly better than MTA in terms of quality of restorative dentin formation. ②At 14 days after operation, iRoot BP Plus had a greater area of neodontinin than that in MTA group(P<0.05). Conclusion Both iRoot BP Plus and MTA can promote the formation of restorative dentin bridges after direct pulp capping, and the formation quality is high. The long-term effects need to be further studied.

Key words: iRoot BP Plus, MTA, direct pulp capping, reparative dentinal

中图分类号:

R783.1

黄彩霞, 张然, 李娇娇, 韦茄彬, 黎淑芳. 应用iRoot BP Plus、MTA直接盖髓后牙髓修复反应变化的大鼠实验研究[J]. 口腔医学, 2025, 45(3): 191-196.

HUANG Caixia, ZHANG Ran, LI Jiaojiao, WEI Jiabin, LI Shufang. Study on the changes in dental pulp repair response in rats after direct pulp capping using iRoot BP Plus and MTA[J]. Stomatology, 2025, 45(3): 191-196.

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分级	定义
修复性硬组织
1	修复性硬组织或牙本质桥覆盖全部穿髓孔
2	穿髓孔处见大量修复性硬组织沉积
3	穿髓孔处见少量修复性硬组织沉积
4	穿髓孔处未见修复性硬组织或牙本质桥生成
牙本质小管
0	修复性硬组织中无牙本质小管存在
1	修复性硬组织中可见牙本质小管,但小管排列不规则
2	修复性硬组织中可见牙本质小管,且小管排列规则

时间	组别	n	硬组织桥组织学评分				平均秩	P	牙本质小管组织学评分			平均秩	P
时间	组别	n	1	2	3	4	平均秩	P	0	1	2	平均秩	P
7 d	iRoot BP Plus	8	0	0	7	1	8.50	0.045^*	6	2	0	14.00	0.334
	MTA	8	0	0	5	3	14.50	0.004^#	7	1	0	12.50
	空白组	8	0	0	1	7	14.50	0.046^&	8	0	0	11.00
14 d	iRoot BP Plus	8	3	4	1	0	6.69	0.003^*	1	6	1	17.25	0.003^*
	MTA	8	1	2	4	1	11.81	0.002^#	5	3	0	11.13	0.036^@
	空白组	7	0	1	0	6	18.29	0.019^&	7	0	0	7.00	0.001^#
28 d	iRoot BP Plus	8	6	2	0	0	7.38	0.001^*	0	2	6	18.13	<0.001^*
	MTA	7	4	2	0	1	9.71	<0.001^#	1	5	1	12.43	0.020^@ <0.001^#
	空白组	8	0	1	1	6	18.63	0.006^&	7	1	0	5.50	0.006^&

分组	7 d	14 d	28 d	F	P
iRoot BP Plus(n=24)	6.40±3.22	13.54±1.75	21.74±2.30	75.671	<0.001^*
MTA(n=23)	4.63±4.00	10.79±1.63	17.41±8.51	10.128	0.004^*
空白(n=23)	0.53±1.49	106.00±2.81	3.36±6.23	0.787	0.480
F	12.398	72.274	19.711	—	—
P	0.001^*	<0.001^*	<0.001^*	—	—