关键词: circRNA_3498, 自噬, 2型糖尿病, 牙周组织, 巨噬细胞

Abstract:

Objective To study the role of circRNA_3498 targeted inhibition of autophagy in the destruction of periodontal tissue in mice with type 2 diabetes mellitus (T2DM). Methods The peripheral blood mononuclear cells from patients with T2DM and healthy volunteers were extracted for transcriptome sequencing and bioinformatics analysis of the differential expression of circRNA. The T2DM mouse model was constructed and the changes of autophagy related protein LC3B, interleukin(IL)-1β and IL-18 in the periodontal tissue structure of mice with T2DM were observed by immunohistochemical staining. Bone marrow-derived macrophages (BMDMs) were isolated from mice. The expression of circRNA_3498 in macrophages was interfered using lentivirus and siRNA. Western blot and ELISA were performed to detect the levels of LC3B, IL-1β, IL-18, as well as macrophage markers CD86 and CD206. Results Compared with the healthy people, circRNA_3498 was highly expressed in PBMCs of patients with T2DM (P<0.05). The expression level of LC3B was reduced in periodontal tissue of mice with T2DM, while IL-1β and IL-18 showed high expression (P<0.05). Lentivirus interference in BMDMs stimulated the expression of circRNA_3498, resulting in a significant decrease in the expression levels of LC3-Ⅱ/LC3-Ⅰ (P<0.05), a reduction in CD206 positive expression (P<0.05), an increase in CD86 positive expression (P<0.05), and an increase in the levels of IL-1β (P<0.01) and IL-18 (P<0.05). After siRNA-circRNA_3498 intervention to inhibit the expression of circRNA_3498, the results were reversed. Conclusion circRNA_3498 promotes inflammation of periodontal tissue by targeting inhibition of autophagy related proteins in mice with T2DM.

Key words: circRNA_3498, autophagy, type 2 diabetes mellitus, periodontal tissue, macrophages