关键词: 10-MDP钙盐, 牙周组织, 基质金属蛋白酶, 细胞凋亡

Abstract:

Objective To investigate the effects of different concentrations of 10-MDP calcium salts on biological behaviors of L929 fibroblasts in the periodontium. Methods The X-Ray diffraction (XRD) was used to examine 10-MDP calcium salts obtained by the three synthesized formulas. The cell proliferation and cytotoxicity of L929 was measured by CCK-8 method; the flow cytometry was used to detect cell apoptosis, and the protein expression of matrix metalloproteinase (MMP)-2 and MMP-9 was measured by Western blot. Results The XRD results confirmed that the 10-MDP calcium salts obtained by the three synthesized formulas were in different structures. In the CCK-8 assay, only the 1.0 mg/mL MDP-1 group caused a decrease in cell proliferation activity after 72 h. No cell apoptosis was induced in each group after 72 hours of culture. The secretion of MMP2 and MMP9 proteins in L929 cells were inhibited in 0.2 mg/mL and 0.1 mg/mL MDP-2 and MDP-1 groups. Conclusion 10-MDP calcium salts may not have a cytotoxic effect on the periodontium; they can exert an inhibitory effect on the destruction of the periodontium by inhibiting the secretion of MMP2 and MMP9.

Key words: 10-MDP calcium salts, periodontium, matrix metalloproteinases, cell apoptosis