唾液细胞外囊泡miRNA-143/145与雌激素缺乏性骨质疏松相关性的临床研究

doi:10.13591/j.cnki.kqyx.2022.06.006

Abstract

Abstract: Objective To investigate the correlation between postmenopausal osteoporosis and the level of miRNA-143/145 derived from extracellular vesicles in saliva, and to explore the diagnostic value of miRNA-143/145 for estrogen deficient osteoporosis. Methods Bone parameters of cancellous bone in premenopausal and postmenopausal female patients undergoing dental implant surgery in our hospital were analyzed by CBCT, and BMD differences were measured. CBCT, serum and saliva samples were collected from premenopausal and postmenopausal women undergoing inpatient surgery in our hospital, and extracellular vesicles were extracted. The morphology of extracellular vesicles in serum and saliva were observed by transmission electron microscopy. The expression level of estrogen in serum was detected by ELISA. qRT-PCR was used to detect miRNA-143/145 levels in extracellular vesicles of serum and saliva. Results After analysis, it was found that the level of estrogen in postmenopausal women decreased significantly, accompanied by a significant decrease in bone mineral density. The extracellular vesicles in serum and saliva observed by transmission electron microscopy (TEM) were cup-shaped bilayer membranous structure about 100 nm in diameter. Compared with premenopausal women, the expression of miRNA-143/145 in blood circulation of postmenopausal women was significantly increased. Further detection of miRNA-143/145 expression in saliva showed a similar trend. Conclusion Results of miRNA-143/145 in extracellular vesicles in saliva are in good agreement with results in serum, which suggests that saliva test, as a noninvasive method, is more convenient for clinicians to diagnose postmenopausal osteoporosis.

Key words: estrogen-deficient osteoporosis, serum, saliva, extracellular vesicle, miRNA-143/145

CLC Number:

R681.4

SHA Sha, LU Lingbo, GUO Peipei, CHEN Shu, JIN Muhan, XU Rongyao. Clinical study on correlation between miRNA-143/145 in saliva and estrogen-deficient osteoporosis[J]. Stomatology, 2022, 42(6): 515-520.

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Clinical study on correlation between miRNA-143/145 in saliva and estrogen-deficient osteoporosis

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