FAM20C在小鼠下颌髁突发育过程中的时空表达

doi:10.13591/j.cnki.kqyx.2022.07.005

Abstract

Abstract: Objective To observe the spatial and temporal expression of FAM20C in condylar cartilage at different stages of condylar development in mice and explore its possible mechanism. Methods HE staining and modified safranin O-fast green staining were used to observe morphological changes of condylar cartilage and subchondral bone in 17.5 days of embryo and 0, 7, and 21 days after birth. Immunohistochemical staining was used to observe the localization and expression of FAM20C in mouse condylar cartilage tissues at corresponding time points. The mean optical density values of FAM20C positive expression were measured and subjected to one-way ANOVA. Results Results of HE staining and modified safranin O-fast green staining showed that with the progression of endochondral ossification, the length of condylar chondrocyte layer decreased; the volume of subchondral bone increased, and the volume of mandibular condyle increased. The immunohistochemical results showed that FAM20C was positively expressed in condylar cartilage tissue of the four groups of mice. FAM20C was mainly expressed in the proliferating chondrocyte layer, and pre-hypertrophic chondrocyte layer of condylar cartilage and a small amount was expressed in the hypertrophic chondrocyte layer and subchondral bone layer. With the development of condyle, the expression of FAM20C gradually decreased. Results of statistical analysis showed that there was a significant difference in FAM20C positive expression at each time point (P<0.01). Conclusion FAM20C is involved in mandibular condyle development in mice and may play an important role in condyle formation by regulating the proliferation and differentiation of condylar chondrocytes.

Key words: FAM20C, condylar cartilage, development, temporal and spatial expression, mice

CLC Number:

R321.53

QI Hao, MA Su, YUAN Zhaoyang, WU Guodong, LIU Peihong. Spatial and temporal expression of FAM20C in mandibular condyle development of mice[J]. Stomatology, 2022, 42(7): 600-603.

References

[1] Shibata S, Suzuki S, Tengan T,et al. A histological study of the developing condylar cartilage of the fetal mouse mandible using coronal sections[J]. Arch Oral Biol, 1996, 41(1):47-54.
[2] Bechtold TE, Kurio N, Nah HD, et al. The roles of Indian hedgehog signaling in TMJ formation[J]. Int J Mol Sci, 2019, 20(24):6300.
[3] Wu YN, Zhang JK, Chen FS. The effects of elevated fibroblast growth factor 23 on mandibular growth in rats[J]. Arch Oral Biol, 2018, 95: 156-164.
[4] 冉春枭. FAM20家族蛋白调控小鼠关节软骨发育过程的机制研究[D].大连:大连医科大学, 2021.
[5] Chen K, Quan HX, Chen G, et al. Spatio-temporal expression patterns of Wnt signaling pathway during the development of temporomandibular condylar cartilage[J]. Gene Expr Patterns, 2017(25/26):149-158.
[6] Palma-Lara I, Pérez-Ramírez M, García Alonso-Themann P, et al. FAM20C overview: Classic and novel targets, pathogenic variants and raine syndrome phenotypes[J]. Int J Mol Sci, 2021, 22(15):8039.
[7] Tagliabracci VS, Wiley SE, Guo X, et al. A single kinase generates the majority of the secreted phosphoproteome[J]. Cell, 2015, 161(7):1619-1632.
[8] Liu PH, Ma S, Zhang H, et al. Specific ablation of mouse Fam20C in cells expressing type I collagen leads to skeletal defects and hypophosphatemia[J]. Sci Rep, 2017, 7(1):3590.
[9] Sheth J, Bhavsar R, Gandhi A,et al. A case of Raine syndrome presenting with facial dysmorphy and review of literature[J]. BMC Med Genet, 2018, 19(1):76.
[10] Yan WJ, Ma P, Tian Y, et al. The importance of a potential phosphorylation site in enamelin on enamel formation[J]. Int J Oral Sci, 2017, 9(11):e4.
[11] Liu C, Zhang H, Jani P,et al. FAM20C regulates osteoblast behaviors and intracellular signaling pathways in a cell-autonomous manner[J]. J Cell Physiol, 2018, 233(4):3476-3486.
[12] Worby CA, Mayfield JE, Pollak AJ, et al. The ABCs of the atypical Fam20 secretory pathway kinases[J]. J Biol Chem, 2021, 296: 100267.
[13] Li H, Jing Y, Zhang R,et al. Hypophosphatemic rickets accelerate chondrogenesis and cell trans-differentiation from TMJ chondrocytes into bone cells via a sharp increase in β-catenin[J]. Bone, 2020, 131: 115151.
[14] Wang XF, Hao JJ, Xie YX, et al. Expression of FAM20C in the osteogenesis and odontogenesis of mouse[J]. J Histochem Cytochem, 2010, 58(11):957-967.
[15] 黄林剑, 邓思敏, 陈军, 等. 纤维软骨和透明软骨不同染色方法的对比研究[J]. 口腔医学, 2018, 38(2):104-108.
[16] Hallett SA, Ono W, Ono N. Growth plate chondrocytes: Skeletal development, growth and beyond[J]. Int J Mol Sci, 2019, 20(23):6009.
[17] Jing Y, Zhou X, Han X,et al. Chondrocytes directly transform into bone cells in mandibular condyle growth[J]. J Dent Res, 2015, 94(12):1668-1675.
[18] Provot S, Schipani E. Molecular mechanisms of endochondral bone development[J]. Biochem Biophys Res Commun, 2005, 328(3):658-665.

Spatial and temporal expression of FAM20C in mandibular condyle development of mice

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