慢性牙周炎通过PERK-CHOP信号通路介导睾周脂肪细胞凋亡的研究

doi:10.13591/j.cnki.kqyx.2022.10.003

Abstract

Abstract: Objective To investigate the effect of chronic periodontitis on apoptosis of rat peritesticular adipocytes through protein kinase R-like ER kinase (PERK)-C/EBP homologous protein (CHOP) signal pathway. Methods Twenty male SD rats were randomly divided into experimental group and control group. Experimental group rat model of chronic periodontitis was established. The bilateral maxillary second molars were ligated with silk thread and injected with Porphyromonas gingivalis-lipopolysaccharide (P. gingivalis-LPS) into the gingival sulcus. The control group was injected with the same amount of PBS into the gingival sulcus. After 3 months of modeling, the rats were killed. The periodontal inflammation was evaluated by measuring the gingival bleeding index (GBI) and the probing pocket depth (PPD). The alveolar bone resorption of the second molar was analyzed by Micro-CT. The apoptosis of peritesticular adipocyte was observed by TUNEL staining. Western blot was used to detect the protein expression levels of PERK, p-PERK, CHOP, Caspase-12 and Cleaved-Caspase-3. Results GBI, PPD and CEJ-ABC in the experimental group were significantly higher than those in the control group (P<0.05), and the rat model of chronic periodontitis was successfully established. The apoptosis rate of peritesticular adipocytes in the experimental group increased significantly (P<0.05), and the expression levels of p-PERK, CHOP, Caspase-12 and Cleaved-Caspase-3 protein increased significantly (P<0.05). Conclusion Chronic periodontitis mediates apoptosis of peritesticular adipocyte in rats through the PERK-CHOP signaling pathway.

Key words: periodontitis, protein kinase R-like ER kinase, C/EBP homologous protein, peritesticular adipocytes, apoptosis

CLC Number:

R781.4

CHEN Yiyan, WU Qianqi, LU Jiayi, GUO Lyuhua. Study on peritesticular adipocytes apoptosis mediated by PERK-CHOP signaling pathway in chronic periodontitis[J]. Stomatology, 2022, 42(10): 878-882.

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Study on peritesticular adipocytes apoptosis mediated by PERK-CHOP signaling pathway in chronic periodontitis

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