Abstract:

Objective To investigate the antibacterial performance and biocompatibility of silver nanoparticles-coated root canal nickel titanium instruments(AgNPs-NiTi). Methods AgNPs-NiTi was prepared using pulse electrochemical deposition. The morphology of AgNPs-NiTi was observed using field emission scanning electron microscopy(FE-SEM), and the elemental composition and content were analyzed using X-ray diffraction(XRD)and energy dispersive spectroscopy(EDS). The mechanical properties of AgNPs-NiTi were tested. After Co-culturing AgNPs-NiTi with E. faecalis, the antibacterial effect was detected by colony-forming units method. By constructing an in vitro model of E. faecalis biofilm in the root canal of teeth, the antibacterial effect of AgNPs-NiTi was observed using FE-SEM and live/dead bacterial staining. In addition, AgNPs-NiTi was co-cultured with Raw 264.7 cells, and its cytotoxicity was detected by CCK-8. Results The pulse electrochemical deposition was used to construct a silver nanoparticle(AgNPs)coating on NiTi instruments with no significant change in the mechanical properties. AgNPs-NiTi significantly inhibited the proliferation of E. faecalis and damaged E. faecalis biofilm in the root canal. AgNPs-NiTi had no significant influence on the proliferation of Raw264.7 cells and had no cytotoxicity. Conclusion The mechanical properties of AgNPs-NiTi are similar to those of nickel titanium instruments. AgNPs-NiTi inhibits E. faecalis proliferation with good biocompatibility.

Key words: root canal nickel titanium instrument, silver nanoparticles(AgNPs)-coated, antibacterial performance, biocompatibility