电纺聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石

Abstract

Abstract: Objective To prepare the electrospun poly (εcaprolactone)/ typeI collagen/ nanophase hydroxyapatite nanofiber,and to examine its characteristics and biocompatibility.The composite material is expected to be used as the biodegradable membrane for guided tissue regeneration.Methods The PCL/COLⅠ and PCL/COLⅠ/nHAP scaffolds were prepared by electrospinning technique and observed by scanning electronic microscope.The morphology,proliferation and differentiation of periodontal ligament cells seeded on the scaffolds were evaluated by scanning electronic microscope,CCK8 assay and realtime quantitative polymerase chain reaction analysis.Results The PCL/COLⅠ and PCL/COLⅠ/nHAP scaffolds had a porous and interwoven structure,while only the PCL/COLⅠ/nHAP showed a rough surface because of the nHAP particles packaged inside the fibers.The results of the scanning electronic microscope and CCK8 assay showed that periodontal ligament cells adhering to the scaffolds extended adequately and proliferated stably.Additionally,the realtime quantitative polymerase chain reaction analysis revealed that runtrelated transcription factor 2 and osteocalcin of the cells cultured on the PCL/COLⅠ/nHAP expressed higher than that of the control group,which suggested that the PCL/COLⅠ/nHAP scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells.Conclusions The PCL/COLⅠ/nHAP scaffold which has good biocompatibility and osteoinductive ability may serve as a novel biodegradable membrane for guided tissue regeneration.

Key words: electrospinning, nanophase hydroxyapatite, guided tissue regeneration

CLC Number:

R780.2

References

[1]Karring T,Nyman S,Lindhe J.Healing following implantation of periodontitis affected roots into bone t2[J].J Clin Periodontol,1980,7(2):96-105
[2]Nyman S,Gottlow J,Karring T,et al.The regenerative potential of the periodontal ligament[J].J Clin Periodontol,1982,9(3):257-265
[3]Chen FM,Zhang J,Zhang M,et al.A review on endogenous regenerative technology in periodontal regenerative medicine[J].Biomaterials,2010,31(31):7892-7927
[4]Gamal AY,Iacono VJ.Enhancing guided t7 regeneration of periodontal defects by using a novel perforated barrier membrane[J].J Periodontol,2013,84(7):905-913
[5]Bottino MC,Thomas V,Schmidt G,et al.Recent advances in the development of GTR/GBR membranes for periodontal regenerationa materials perspective[J].Dent Mater,2012,28(7):703-721
[6]李好义，刘勇，何雪涛，等.应用于组织工程支架制备的电纺技术[J].生物工程学报,2012,28(1):15-25
[7]Szentivanyi AL,Zernetsch H,Menzel H,et al.A review of developments in electrospinning technology:new opportunities for the design of artificial t10 structures[J].Int J Artif Organs,2011,34(10):986-997
[8]Shuakat MN,Lin T.Recent developments in electrospinning of nanofiber yarns[J].J Nanosci and Nanotechnol,2014,14(2):1389-1408
[9]Zakaria SM,Sharif Zein SH,Othman MR,et al.Nanophase hydroxyapatite as a biomaterial in advanced hard t5 engineering:a review[J].T5 Eng Part B Revi,2013,19(5):431-441
[10]Fox K,Tran PA,Tran N.Recent advances in research applications of nanophase hydroxyapatite[J].Chemphyschem,2012,13(10):2495-2506
[11]Sullivan M,Mchale K,Parvizi J,et al.Nanotechnology current concepts in orthopaedic surgery and future directions[J].Bone Joint J,2014,96(5):569-573
[12]Balasundaram G,Webster TJ.Nanotechnology and biomaterials for orthopedic medical applications[J].Nanomedicine,2006,1(2):169-176
[13]Livak KJ,Schmittgen TD.Analysis of relative gene expression data using realtime quantitative PCR and the method[J].Methods,2001,25(4):402-408
[14]Ricci G,Rasperini G,Silvestri M,et al.In vitro permeability evaluation and colonization of membranes for periodontal regeneration by Porphyromonas gingivalis[J].J Periodontol,1996,67(5):490-496
[15]Shue L,Yufeng Z,Mony U.Biomaterials for periodontal regeneration:a review of ceramics and polymers[J].Biomatter,2012,2(4):271-277
[16]Wang HL,Modarressi M,Fu JH.Utilizing collagen membranes for guided t1 regenerationbased root coverage[J].Periodontology 200,2012,59(1):140-157
[17]Kikuchi M.Hydroxyapatite/collagen bonelike nanocomposite[J].Biol and Pharm Bull,2013,36(11):1666-1669
[18]Swetha M,Sahithi K,Moorthi A,et al.Biocomposites containing natural polymers and hydroxyapatite for bone t1 engineering[J].Int J Biol Macromol,2010,47(1):1-4
[19]StoecklinWasmer C,Rutjes A,Da Costa B,et al.Absorbable collagen membranes for periodontal regeneration a systematic review[J].J Dent Res,2013,92(9):773-781
[20]Ferreira AM,Gentile P,Chiono V,et al.Collagen for bone t9 regeneration[J].Acta Biomater,2012,8(9):3191-3200
[21]Dash TK,Konkimalla VB.Polyεcaprolactone based formulations for drug delivery and t1 engineering:A review[J].J Control Release,2012,158(1):15-33
[22]Ravichandran R,Sundarrajan S,Venugopal JR,et al.Advances in polymeric systems for t3 engineering and biomedical applications[J].Macromol Biosci,2012,12(3):286-311
[23]Dash TK,Konkimalla VB.Polymeric modification and its implication in drug delivery:polyεcaprolactone (PCL) as a model polymer[J].Mol Pharm,2012,9(9):2365-2379
[24]Pohlmann AR,Fonseca FN,Paese K,et al.Poly (εcaprolactone) microcapsules and nanocapsules in drug delivery[J].Expert Opinion Drug Deliv,2013,10(5):623-638
[25]Enomoto H,Furuichi T,Zanma A,et al.Runx2 deficiency in chondrocytes causes adipogenic changes in vitro[J].J Cell Sci,2004,117(Pt 3):417-425
[26]SilaAsna M,Bunyaratvej A,Maeda S,et al.Osteoblast differentiation and bone formation gene expression in strontiuminducing bone marrow mesenchymal stem cell[J].Kobe J Med Sci,2007,53(1/2):25-35
[27]Stein GS,Lian JB,Owen TA.Relationship of cell growth to the regulation of t13specific gene expression during osteoblast differentiation[J].FASEB J,1990,4(13):3111-3123
[28]Malaval L,Liu F,Roche P,et al.Kinetics of osteoprogenitor proliferation and osteoblast differentiation in vitro[J].J Cell Biochemi,1999,74(4):616-627

Preparation and biocompatibility of electrospun poly (εcaprolactone)/ typeI collagen/ nanophase hydroxyapatite composite fibrous scaffold

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