p27在调节小鼠下颌牙槽骨生长发育中的作用

Abstract

Abstract: Objective: To explore the mechanism of action of the p27kip1 in intramembranous ossification. Methods: The phenotypes of the mandible were compared between p27kip1 gene knockout (p27-/-) mice and their wild-type littermates at 2 weeks of age by radiograph, histochemistry and immunohistochemistry. Sonic Hedgehog (Shh) pathway is analyzed by Western blot. Results: Compared with wild-type mice, the volume and mineral density was increased in the mandibles. Both the alkaline phosphatase positive areas and type I collagen immunopositive areas were increased significantly in the mandibles, whereas protein expression levels of Shh, Patched, Smoothened and Gli2 were up-regulated in mandibles from p27-/- mice compared with those from wild-type mice. Conclusion: Results from this study indicate that p27 plays a negative regulatory role in alveolar bone development by inhibiting shh signal pathway and cell proliferation. This study elucidates the role and mechanism of p27 in regulating tooth and mandible development, thus provide experimental and theoretical basis for p27 as a new target for the growth and development of tooth and mandible.

Key words: p27, Alveolar bone, development

References

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p27kip1 Regulates Alveolar Bone Formation in Postnatal Mice

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