载银锌介孔钙硅纳米粒子与聚己内酯复合材料的体外生物活性研究

doi:10.13591/j.cnki.kqyx.2022.02.003

Abstract

Abstract: Objective To investigate the physical and chemical properties, biological activity in vitro and pro-osteogenic properties of poly-ε-caprolactone (PCL) and mesoporous calcium silicate nanoparticles (MCSNs) containing silver or zinc composite materials. Methods The composite materials of PCL-MCSNs, PCL-Zn-MCSNs, PCL-Ag-MCSNs, PCL-Ag-Zn-MCSNs were prepared by heating and melting method and characterized to test their surface morphology, mechanical properties and degradation properties in vitro. The composite materials were co-cultured with stem cells from the apical papilla (SCAP) to observe cell adhesion and hydroxyapatite (HA) formation on the surface. The cytotoxicity of composite materials and pro-osteogenic properties in vitro were studied by CCK-8 method, alkaline phosphatase (ALP) activity determination and Alizarin Red staining. Results The surface of each group was smooth and even. The compressive strength of the PCL-MCSNs group was 3.05 MPa, which was the highest. PCL-Zn-MCSNs showed the most HA formation, the fastest degradation, the highest surface cell density and the best cell adhesion. Except for PCL-Ag-MCSNs and PCL-Ag-Zn-MCSNs, all the experimental groups could promote cell proliferation, increase ALP activity, and produce more calcified nodular content than PCL, among which PCL-Zn-MCSNs was significantly higher than the other groups (P<0.05). Conclusion The composite materials of PCL and MCSNs containing silver or zinc reveal good mechanical and degradation properties. The cell adhesion, cell proliferation, ALP activity and calcified nodule formation ability are enhanced with the increase of Zn content, suggesting that this kind of composite materials possess good biological activity in vitro and superior pro-osteogenic/odontogenic potential. These materials are expected to be a new material for the repair of oral and maxillofacial bone defects.

Key words: mesoporous calcium silicon nanoparticles, bone tissue engineering, polycaprolactone, apical papilla stem cell

CLC Number:

R783.1

WANG Ying, LI Mingming, ZHAO Xiao, LENG Diya, WU Daming. In vitro biological activity study on the material composited with nanosilver and nanozinc incorporated mespoporous calcium-silicate and PCL[J]. Stomatology, 2022, 42(2): 110-116.

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In vitro biological activity study on the material composited with nanosilver and nanozinc incorporated mespoporous calcium-silicate and PCL

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