Abstract:

Objective A sustained release system of titanium dioxide nanotubearray loaded with Ebselen(EB) was constructed on a titanium surface to investigate the kinetics of drug release and its effect on osteoblast behaviors. Methods Titanium dioxide nanotubes(TiO₂ nanotubes, TNT) with length of 2 μm and diameter of 120 nm was prepared on titanium substrate by anodic oxidation, and then EB of different mass was loaded by freeze-drying method. The micromorphology, surface element composition and drug release characteristics of the nanotube orifice were observed and analyzed. MC3T3-E1 osteoblasts were inoculated in different concentrations of EB release solutions, and the optimal loading amount of TNT loaded EB was explored on the basis of micro-nano morphology, osteogenic properties and release kinetic characteristics. Results The selenium element could be detected after loading EB on TNT prepared on pure titanium substrate. When the EB loaded on TNT was less than 5 μg/cm², the TNT array could be completely retained and different amounts of EB could be completely released after 24 h. When the EB concentration was less than 10 μmol/L, the proliferation and osteogenic differentiation activities of MC3T3-E1 were promoted, among which, 5 μmol/L group showed the best effect. Conclusion When the EB loaded on TNT is below 5 μg/cm², the nanotube topological structure remains intact, and the best in vitro osteogenic activity is achieved at the EB concentration of 5 μmol/L.

Key words: TiO₂ nanotube, Ebselen, drug delivery, osteoblast