TGF-β3对成骨细胞的增殖和成骨能力的影响及其机制初探

doi:10.13591/j.cnki.kqyx.2022.06.004

Abstract

Abstract: Objective To explore the effect of transforming growth factor-β3 (TGF-β3) in different doses on the proliferation and osteogenic capability of osteoblasts and its mechanism. Methods Osteoblasts were isolated from the calvarium of neonatal New Zealand white rabbit, purified and identified and induced by different doses (0.1, 1, 10, 100 μg/L) of TGF-β3. Cell proliferation of each group was tested by CCK-8 assay and ALP activity was measured. The expression levels of COL-1A1, Runx-2 and OCN proteins were examined by immunohistochemical staining method. Relative expression of ALP, OPN, BSP, Osx and Smad4 genes were measures by qPCR. Western blotting method was used to test the expression of Smad2/3 protein. Results The osteoblasts were isolated and identified successfully. 10 μg/L and 100 μg/L TGF-β3 can improve the proliferation of osteoblasts. The ALP activity in 10 μg/L group was higher than the control group (P＜0.05). Immunohistochemical staining results showed the protein expression in 10 μg/L and 100 μg/L TGF-β3 group was stronger than the control group. The ALP, OPN, BSP, Osx and Smad4 gene expression in 10 μg/L and 100 μg/L TGF-β3 group was higher than the control group (P＜0.05). The Smad2/3 protein expression in 1-100 μg/L TGF-β3 group was higher than the control group (P＜0.05). Conclusion TGF-β3 10-100 μg/L can improve the proliferation and osteogenic ability of osteoblasts and this effect is more obvious when the dose is 10 μg/L. This effect is produced through activating Smad-dependent pathway.

Key words: transforming growth factor-β3 (TGF-β3), osteoblast, proliferation, osteogenic capability, Smad-dependent pathway

CLC Number:

R782

AINIWAER Ailimaierdan, HUOJIA Muhetaer, WANG Ling. The effect and primary mechanism of transforming growth factor-β3 on the proliferation and osteogenic capability of osteoblasts[J]. Stomatology, 2022, 42(6): 501-507.

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The effect and primary mechanism of transforming growth factor-β3 on the proliferation and osteogenic capability of osteoblasts

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