氟西汀单独使用及联用氟康唑对白念珠菌的影响研究

doi:10.13591/j.cnki.kqyx.2024.05.005

Abstract

Abstract:

Objective To evaluate the inhibitory activity of fluoxetine alone and in combination with fluconazole on Candida albicans. Methods This study used standard strains of Candida albicans, clinical isolates of Candida albicans, and drug-resistant strains of Candida albicans for experiments to prepare the planktonic and biofilm states of Candida albicans, respectively. The effect of fluoxetine on Candida albicans was detected by XTT reduction method. Pharmacodynamic properties of fluoxetine against Candida albicans biofilm were determined by improved time-kill test. The morphological changes of fluoxetine on Candida albicans biofilm were observed by scanning electron microscopy, and the synergistic effect of fluoxetine combined with fluconazole on Candida albicans biofilms was detected by the checkerboard dilution method. Results Fluoxetine had inhibitory effects on three types of Candida albicans biofilms. The minimum drug concentration that inhibited 50% activity (SMIC₅₀) of biofilms of standard strains of Candida albicans and drug-resistant strains was 32 μg/mL; the SMIC₅₀ of clinical strains of Candida albicans biofilm was 64 μg/mL. Time-kill test displayed that the biofilm activity of three types of Candida albicans significantly decreased compared to the control group at the fluoxetine concentration of 1×SMIC₅₀ and 2×SMIC₅₀. Scanning electron microscopy observation showed that compared with the control group, the number of fungal hyphae in the biofilm of Candida albicans treated with fluoxetine decreased and their morphology wrinkled. The checkerboard microdilution method showed that the combination of fluoxetine and fluconazole exhibited a synergistic effect on the standard strain of Candida albicans bioflim, while showing no or antagonistic effects on the other two strains. Conclusion Fluoxetine alone has inhibitory effects on biofilms of different types of Candida albicans and has a synergistic inhibitory effect on biofilms of standard strains of Candida albicans combined with fluconazole.

Key words: Candida albicans, fluoxetine, fluconazole

CLC Number:

R781.5

SHI Banruo, WU Qiaochu, MIAO Haochen, WEI Xin. Study on the effect of fluoxetine against Candida albicans, alone or combined with fluconazole[J]. Stomatology, 2024, 44(5): 344-348.

Figures/Tables 4

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References 26

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白念珠菌菌株	氟康唑	氟西汀
标准株
浮游状态MIC₈₀	0.5	128.0
生物膜状态SMIC₅₀	512.0	32.0
临床分离株
浮游状态MIC₈₀	4.0	128.0
生物膜状态SMIC₅₀	1 024.0	64.0
氟康唑耐药株
浮游状态MIC₈₀	4.0	128.0
生物膜状态SMIC₅₀	1 024.0	32.0

菌株	FICI法				ΔE法
菌株	FICI		结果		∑SYN/%		∑ANT/%		结果
白念珠菌标准株		0.38		SYN		701		-36		SYN
临床分离株		0.75		IND		65		-218		ANT
氟康唑耐药株		1.50		IND		13		-1 078		ANT

Study on the effect of fluoxetine against Candida albicans, alone or combined with fluconazole

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