ROS响应型纳米载体用于牙髓炎症的抗氧化治疗

doi:10.13591/j.cnki.kqyx.2025.07.006

摘要/Abstract

摘要：

目的探究掺铈介孔生物活性玻璃纳米颗粒(Cerium-containing mesoporous bioactive glass nanoparticles,Ce-MBGNs)作为活性氧(reactive oxygen species,ROS)响应型纳米载体对牙髓炎症的影响,及其促进牙髓干细胞(dental pulp stem cells,DPSCs)成牙本质分化的潜力。方法 CCK-8检测Ce-MBGNs的细胞毒性,RT-qPCR检测其对DPSCs成骨/成牙本质分化相关mRNA以及对RAW264.7炎症因子表达的影响;碱性磷酸酶及茜素红S染色研究其促进牙本质再矿化的能力。免疫荧光及酶标仪检测Ce-MBGNs清除ROS的能力,JC-1染色检测线粒体膜电位。结果 Ce-MBGNs生物相容性良好,可显著提高DPSCs中成骨/成牙本质相关基因表达,促进矿化。同时Ce-MBGNs有效清除细胞内ROS,维持线粒体膜电位,抑制促炎因子表达,促进抗炎因子表达。结论 Ce-MBGNs可以通过维持线粒体膜电位,控制炎症,保护DPSCs免受自身氧化损伤,并促其成牙本质分化,为开发口腔新型治疗药剂提供了理论基础。

关键词: 掺铈介孔生物活性玻璃, 牙髓干细胞, 免疫调节, 成骨/牙本质向分化

Abstract:

Objective To explore the effects of cerium-doped mesoporous bioactive glass nanoparticles (Ce-MBGNs) as reactive oxygen species (ROS)-responsive nanocarriers on inflammation in pulp and their potential to promote odontogenic differentiation of dental pulp stem cells (DPSCs). Methods The cytotoxicity of Ce-MBGNs was assessed by CCK-8. The expression of mRNA of osteogenic/odontogenic differentiation in DPSCs and that of inflammatory factor in RAW264.7 cells were detected by RT-qPCR. Alkaline phosphatase and Alizarin Red S staining were utilized to investigate their ability to promote dentin mineralization. The ability of Ce-MBGNs to scavenge ROS was evaluated by immunofluorescence and JC-1 staining was used to assess the membrane potential (MMP) of mitochondrial. Results Ce-MBGNs exhibited good biocompatibility and significantly increased the expression of osteogenic/odontogenic-related genes in DPSCs, promoting mineralization. Additionally, Ce-MBGNs effectively scavenged intracellular ROS, maintained MMP, inhibited the expression of pro-inflammatory factors, and promoted the expression of anti-inflammatory factors. Conclusion Ce-MBGNs can protect DPSCs from oxidative damage by maintaining the MMP of mitochondrial and controlling inflammation, and promote their odontoblastic differentiation, providing a theoretical basis for the development of novel therapeutic agents for oral treatment.

Key words: Ce-MBGNs, dental pulp stem cells, immunomodulation, osteogenic/odontoblastic differentiation

中图分类号:

R780.2

王铭心, 段伊媛, 冯可嘉, 聂晨瑜, 马骞. ROS响应型纳米载体用于牙髓炎症的抗氧化治疗[J]. 口腔医学, 2025, 45(7): 511-517.

WANG Mingxin, DUAN Yiyuan, FENG Kejia, NIE Chenyu, MA Qian. A novel ROS-responsive nanocarrier for antioxidant therapy in endodontic inflammation[J]. Stomatology, 2025, 45(7): 511-517.

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基因名称	引物序列
ALP	Forward:5'-AACATCAGGGACATTGACGTG-3' Reverse:5'-GTATCTCGGTTTGAAGCTCTTCC-3'
DSPP	Forward:5'-GGGAATAGAAATCAAGGGTC-3' Reverse:5'-CAAGATCATTCCATGTTGTCC-3'
DMP-1	Forward:5'-GGTATCACACCCAACTATGAAGATCA-3' Reverse:5'-CCATCATACTGAGCAGCAAAGTTC-3'
BMP2	Forward:5'-ACCCGCTGTCTTCTAGCGT-3' Reverse:5'-TTTCAGGCCGAACATGCTGAG-3'
GAPDH	Forward:5'-CAAGGTCATCCATGACAACTTTG-3' Reverse:5'-GTCCACCACCCTGTTGCTGTAG-3'

基因名称	引物序列
IL-6	Forward:5'-CTGCAAGAGACTTCCATCCAG-3' Reverse:5'-AGTGGTATAGACAGGTCTGTTGG-3'
IL-10	Forward:5'-TCAAGGCGCATGTGAACTCC-3' Reverse:5'-GATGTCAAACTCACTCATGGCT-3'
GAPDH	Forward:5'-CCGAGACCAACCGAGTCATTTA-3' Reverse:5'- GATGGTGATGGGTTTCCCGT-3'