甘草多糖抑制唾液腺腺样囊性癌生长和迁移能力的研究

doi:10.13591/j.cnki.kqyx.2025.11.005

摘要/Abstract

摘要：

目的探讨甘草多糖对唾液腺腺样囊性癌肿瘤细胞生物学行为的影响及其抗肿瘤的作用机理。方法制备甘草多糖母液,以人腺样囊性癌高转移细胞系SACC-LM和低转移细胞系SACC-83为靶细胞,用不同浓度的甘草多糖进行干预。采用CCK-8实验检测细胞增殖和凋亡情况;RT-PCR和Western Blot实验分别检测肿瘤侵袭转移相关基因和蛋白的表达;划痕实验观察甘草多糖对SACC细胞迁移能力的影响;动物实验验证甘草多糖对肿瘤细胞生长的抑制作用。结果甘草多糖可显著抑制人唾液腺腺样囊性癌高转移细胞系的增殖和迁移能力,并通过调节上皮-间充质转化相关标志物的表达,抑制其恶性生物学行为;体内实验显示,甘草多糖无明显肝毒性,可显著降低瘤体体积和质量,并抑制裸鼠瘤体的生长速度。结论甘草多糖对SACC的生长及迁移能力有一定的抑制作用。

关键词: 唾液腺腺样囊性癌, 甘草多糖, 迁移

Abstract:

Objective To explore the effects of glycyrrhizic polysaccharide(GP) on the biological activity of salivary adenoid cystic carcinoma tumor cells and its mechanisms of anti-tumor action. Methods GP was prepared, and human adenoid cystic carcinoma high-metastatic cell line SACC-LM and low-metastatic cell line SACC-83 were used as target cells. Different concentrations of GP were used for intervention. The cell counting kit-8 (CCK-8) assay was employed to detect cell proliferation and apoptosis. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot experiments were conducted to detect the expression of genes and proteins related to tumor invasion and metastasis. The scratch assay was used to observe the effect of GP on the migration ability of SACC cells. In vivo experiments were conducted to verify the inhibitory effect of GP on tumor cells. Results Glycyrrhiza polysaccharides significantly inhibited the proliferation and migration of the highly metastatic human adenoid cystic carcinoma cell line SACC-LM by regulating the expression of epithelial-mesenchymal transition (EMT)-related markers. In vivo experiments showed that glycyrrhiza polysaccharides had no significant hepatotoxicity, could significantly reduce the volume and weight of tumors, and inhibit the growth rate of tumors in nude mice. Conclusion GP has a certain inhibitory effect on the growth and migration ability of SACC.

Key words: salivary adenoid cystic carcinoma, glycyrrhizic polysaccharide, migration

中图分类号:

R780.2

刘唐君, 单天宇, 王馨伟, 孙丹丹, 刘伽伽, 柳云霞. 甘草多糖抑制唾液腺腺样囊性癌生长和迁移能力的研究[J]. 口腔医学, 2025, 45(11): 826-831.

LIU Tangjun, SHAN Tianyu, WANG Xinwei, SUN Dandan, LIU Jiajia, LIU Yunxia. Research on the inhibition of glycyrrhizic polysaccharide on the growth and migration of salivary adenoid cystic carcinoma cells[J]. Stomatology, 2025, 45(11): 826-831.

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基因名称	上游引物(5'-3')	下游引物(5'-3')
BTBD7	AGTCAAATGCCTGGTTACGG	TGTCTGGCACATTGGACATT
FN	GAGAATAAGCTGTACCATCGCAA	CGACCACATAGGAAGTCCCAG
E-cadherin	ATTTTTCCCTCGACACCCGAT	TCCCAGGCGTAGACCAAGA
Vimentin	GGCGAGGAGAGCAGGATTTC	TGGGTATCAACCAGAGGGAGT
β-actin	CTGCAATCCGAAAGAAGCTG	ATCTTCAAACCTCCATGATG