Claudin-7在口腔鳞状细胞癌中的表达和作用研究

doi:10.13591/j.cnki.kqyx.2022.07.006

口腔医学 ›› 2022, Vol. 42 ›› Issue (7): 604-608.doi: 10.13591/j.cnki.kqyx.2022.07.006

Claudin-7在口腔鳞状细胞癌中的表达和作用研究

王金鑫¹, 伊婕¹, 丁宇洁¹, 钟旖², 孙志达¹

1 南京医科大学附属口腔医院牙体牙髓科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京(210029);
2 南京医科大学附属口腔医院口腔病理科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京(210029)

修回日期:2022-03-29 出版日期:2022-07-28 发布日期:2022-07-22
通讯作者: 孙志达 E-mail:suzhida@njmu.edu.cn

Expression and role of Claudin-7 in oral squamous cell carcinoma

WANG Jinxin, YI Jie, DING Yujie, ZHONG Yi, SUN Zhida

Department of Endodontics, Affiliated Stomatological Hospital of Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China

Revised:2022-03-29 Online:2022-07-28 Published:2022-07-22

摘要/Abstract

摘要： 目的探究Claudin-7在口腔鳞状细胞癌(OSCC)中的表达及作用。方法通过免疫组化实验和蛋白质免疫印记实验检测OSCC中Claudin-7的表达情况,并分析Claudin-7和不同病理参数的关系。通过慢病毒转染Cal27和HN6细胞系上调Claudin-7表达,通过CCK-8和Transwell实验检测细胞增殖及迁移能力的变化;通过蛋白质免疫印迹实验和实时荧光定量聚合酶链式反应(RT-qPCR)检测Claudin-7对上皮间充质转化(EMT)指标的影响。结果 Claudin-7在OSCC中低表达(P<0.05),其表达水平和OSCC患者肿瘤大小、分化程度和淋巴结转移相关(P<0.05)。过表达Claudin-7可抑制Cal27和HN6细胞增殖和迁移,并且上调E-钙黏蛋白(E-cadherin)及下调N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)的蛋白和mRNA(P<0.05)。结论 Claudin-7在OSCC中低表达,上调其表达可通过影响上皮间充质转化抑制 OSCC细胞的增殖和迁移。Claudin-7可作为OSCC治疗的一个新靶点。

关键词: Claudin-7, 口腔鳞状细胞癌, 上皮间充质转化, 增殖, 迁移

Abstract: Objective To explore the expression and role of Claudin-7 in oral squamous cell carcinoma (OSCC). Methods Expression of Claudin-7 in OSCC was detected by immunohistochemistry experiment and western blot experiment, and the relationship between Claudin-7 and pathological parameters was analyzed. Lentiviral was used to transfect Cal27 and HN6 cell line to increase the expression of Claudin-7. CCK-8 assay and Transwell assay were used to detect changes in cell proliferation and migration ability. Western blot experiments and RT-qPCR experiments were used to detect the effect of up-regulated Claudin-7 on the index of epithelial-mesenchymal transition. Results Claudin-7 was decreased in OSCC (P<0.05) and its expression level was related to patient′s tumor size, differentiation degree and lymph node metastasis (P<0.05). Overexpression of Claudin-7 could inhibit the proliferation and migration of Cal27 and HN6 cells, up-regulate the expression of E-cadherin protein and mRNA, and down-regulate the expression of N-cadherin and Vimentin protein and mRNA (P<0.05). Conclusion Claudin-7 is expressed at low levels in OSCC. Up-regulating the expression of Claudin-7 can inhibit the proliferation and migration of OSCC cells by regulating epithelial-mesenchymal transition. Claudin-7 can be used as a new target for the treatment of OSCC.

Key words: Claudin-7, oral squamous cell carcinoma, epithelial-mesenchymal transition, proliferation, migration

中图分类号:

R739.8

王金鑫, 伊婕, 丁宇洁, 钟旖, 孙志达. Claudin-7在口腔鳞状细胞癌中的表达和作用研究[J]. 口腔医学, 2022, 42(7): 604-608.

WANG Jinxin, YI Jie, DING Yujie, ZHONG Yi, SUN Zhida. Expression and role of Claudin-7 in oral squamous cell carcinoma[J]. Stomatology, 2022, 42(7): 604-608.

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Claudin-7在口腔鳞状细胞癌中的表达和作用研究

Expression and role of Claudin-7 in oral squamous cell carcinoma

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