人参皂苷Rg1对人牙龈成纤维细胞增殖、迁移及炎症因子表达的影响研究

doi:10.13591/j.cnki.kqyx.2025.11.008

摘要/Abstract

摘要：

目的探讨人参皂苷(ginsenosides Rg1,GSRG1)对脂多糖(lipopolysaccharide,LPS)刺激下人牙龈成纤维细胞(human gingival fibroblasts,HGFs)增殖、迁移以及炎症因子表达的影响及机制研究。方法采用组织块法分离培养HGFs,ELISA法检测不同浓度(0、1、5、10、20 μg/mL)LPS对HGFs炎症细胞因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)的影响。将细胞分为正常对照组(未进行处理)、LPS组(加入20 μg/mL LPS)和LPS+GSRG1组(同时加入20 μg/mL LPS和100 mg/L GSRG1),CCK-8检测各组HGFs细胞增殖,Transwell实验观察迁移能力,流式细胞仪比较各组细胞内活性氧(reactive oxygen species,ROS)水平,Western blot检测TNF-α、IL-6、NOD样受体家族热蛋白结构域相关蛋白3(NOD-like receptor family pyrin domain-containing protein 3,NLRP3)、p-p38与p38蛋白表达水平。结果 LPS浓度为5、10、20 μg/mL时,HGFs细胞中IL-6、TNF-α表达均明显增加(P<0.05),其中20 μg/mL水平最高。与正常对照组相比,LPS组HGFs在第1、2天时细胞增殖变化不明显,在第3、4、5天时增殖减少,迁移能力下降,ROS水平明显增加(P<0.001),TNF-α、IL-6、NLRP3蛋白表达明显增加(P<0.001),p-p38蛋白表达明显减少(P<0.001);与LPS组相比,LPS+GSRG1组细胞增殖和迁移能力显著增加(P<0.05),ROS水平明显减少,TNF-α、IL-6、NLRP3蛋白表达减少,p-p38蛋白表达增多(P<0.001);三组间p38表达无明显变化。结论 GSRG1可改善LPS对HGFs增殖和迁移的抑制作用,减轻炎症反应,其机制可能与p-p38调控有关。

关键词: 人牙龈成纤维细胞, 人参皂苷Rg1, 细胞增殖, 细胞迁移, 炎症

Abstract:

Objective To investigate the effects and molecular mechanisms of ginsenoside Rg1 (GSRG1) on the proliferation, migration, and inflammatory cytokine expression of human gingival fibroblasts (HGFs) induced by lipopolysaccharide (LPS). Methods HGFs were isolated and cultured using the tissue block method. The effects of different concentrations of LPS (0, 1, 5, 10, 20 μg/mL) on inflammatory cytokines in HGFs were detected by ELISA. Cells were divided into three groups: control group (no treatment), LPS group (20 μg/mL LPS), and LPS+GSRG1 group (20 μg/mL LPS and 100 mg/L GSRG1). Cell proliferation was detected by cell counting kit-8 (CCK-8); cell migration was assessed by Transwell assay; intracellular reactive oxygen species (ROS) levels were compared using flow cytometry; and the expression levels of TNF-α, IL-6, NLRP3, p-p38, and p38 proteins were detected by Western blot. Results LPS concentrations of 5, 10, and 20 μg/mL significantly increased the expression of IL-6 and TNF-α in HGFs (P<0.05), with 20 μg/mL showing the strongest pro-inflammatory effect. Compared with the control group, there was no notable difference in the proliferation of HGFs in the LPS group at Day 1 and 2. However, on Day 3, 4 and 5, decreased cell proliferation, reduced migration, significantly increased ROS levels (P<0.001), elevated protein expression of TNF-α, IL-6, and NLRP3 (P<0.001), and reduced p-p38 protein expression (P<0.001) were exhibited. Compared with the LPS group, the LPS+GSRG1 group showed significantly enhanced cell proliferation and migration (P<0.05), reduced ROS levels, decreased protein expression of TNF-α, IL-6, and NLRP3, and increased p-p38 protein expression (P<0.001). There was no significant change in p38 expression among the three groups. Conclusion GSRG1 can alleviate the inhibitory effects of LPS on the proliferation and migration of HGFs and inhibit the inflammatory response, potentially through mechanisms involving p-p38 protein regulation.

Key words: human gingival fibroblasts, ginsenoside Rg1, cell proliferation, cell migration, inflammation

中图分类号:

R781.4

曹倩, 袁长永, 周瑶, 李晓飞, 朱绍跃, 王鹏来. 人参皂苷Rg1对人牙龈成纤维细胞增殖、迁移及炎症因子表达的影响研究[J]. 口腔医学, 2025, 45(11): 844-848.

CAO Qian, YUAN Changyong, ZHOU Yao, LI Xiaofei, ZHU Shaoyue, WANG Penglai. Effects of ginsenoside Rg1 on proliferation, migration and inflammatory cytokine expression in human gingival fibroblasts[J]. Stomatology, 2025, 45(11): 844-848.

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