关键词: 口腔黏膜, 创伤愈合, 再上皮化, 初级纤毛, 衰老

Abstract:

Objective To investigate the correlation between ageing changes in primary cilia and oral mucosal wound healing. Methods A wound model was constructed for buccal oral mucosa of mice at different ages, and samples were collected at 1, 3 and 5 days after modeling. The healing process was observed by HE staining, the tissue remodeling by MASSON staining, the re-epithelialization process by immunofluorescence staining, and the difference of cell proliferation level during the wound healing process by EdU labeling. By constructing mice with specific knockout of the primary cilia key molecule Ift140 in oral mucosal epithelial, the difference of oral mucosal wound healing speed between knockout and control mice was observed. Results The oral mucosal epithelium of aged mice had decreased self-renewal ability and the occurrence rate of primary cilia was reduced. Mucosal healing was delayed after wound, and the proliferation rate of keratinocytes was reduced and the migration rate to the wound bed was slowed. Conditional knockdown of Ift140 in oral mucosal epithelial K14-positive cells interfered with primary cilia formation and delayed the wound healing process. Conclusion Age-related changes in primary cilia of mouse oral mucosal epithelium delay oral mucosal wound healing.

Key words: oral mucosa, wound healing, re-epithelialization, primary cilia, aging